Publication

Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents

Haegens, A., Vernooy, J. H. J., Heeringa, P., Mossman, B. T. & Wouters, E. F. M., Feb-2008, In : European Respiratory Journal. 31, 2, p. 252-260 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Haegens, A., Vernooy, J. H. J., Heeringa, P., Mossman, B. T., & Wouters, E. F. M. (2008). Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents. European Respiratory Journal, 31(2), 252-260. https://doi.org/10.1183/09031936.00029307

Author

Haegens, A. ; Vernooy, J. H. J. ; Heeringa, P. ; Mossman, B. T. ; Wouters, E. F. M. / Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents. In: European Respiratory Journal. 2008 ; Vol. 31, No. 2. pp. 252-260.

Harvard

Haegens, A, Vernooy, JHJ, Heeringa, P, Mossman, BT & Wouters, EFM 2008, 'Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents' European Respiratory Journal, vol. 31, no. 2, pp. 252-260. https://doi.org/10.1183/09031936.00029307

Standard

Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents. / Haegens, A.; Vernooy, J. H. J.; Heeringa, P.; Mossman, B. T.; Wouters, E. F. M.

In: European Respiratory Journal, Vol. 31, No. 2, 02.2008, p. 252-260.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Haegens A, Vernooy JHJ, Heeringa P, Mossman BT, Wouters EFM. Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents. European Respiratory Journal. 2008 Feb;31(2):252-260. https://doi.org/10.1183/09031936.00029307


BibTeX

@article{42a8cc0c111a4aea8b836219b5e67254,
title = "Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents",
abstract = "During extensive inflammation, neutrophils undergo secondary necrosis causing myeloperoxidase (MPO) release that may damage resident lung cells. Recent observations suggest that MPO has pro-inflammatory properties, independent of its enzymatic activity. The aims of the present study were to characterise MPO internalisation by lung epithelial cells and to investigate the effect of MPO on oxidative stress, DNA damage and cytokine production by lung epithelial cells.Human alveolar and bronchial epithelial cells were stimulated with MPO, with or without priming the cells with pro-inflammatory stimuli. MPO protein was detected in the cell cytoplasm. Expression of haernoxygenase (HO)-1 and DNA strand breakage were determined. The production of interleukin (IL)-8 and -6 were measured.Analyses of MPO-stimulated cells demonstrated MPO presence in the cells. HO-1 expression was increased after MPO stimulation and increased further when cells were primed before MPO stimulation. MPO exposure also induced DNA strand breakage. Interestingly, MPO inhibited IL-8 production in bronchial, but not alveolar epithelium.In conclusion, alveolar and bronchial epithelial cells can internalise myeloperoxidase. Stimulation with myeloperoxidase increases haemoxygenase-1 expression and DNA strand breakage, suggesting cell damaging capacity of myeloperoxidase. In addition, myeloperoxidase inhibited interleukin-8 production by bronchial epithelial cells, indicating a negative feedback loop for neutrophil recruitment.",
keywords = "inflammation, myeloperoxidase, neutrophilia, oxidative stress, NF-KAPPA-B, GENE-EXPRESSION, HEME OXYGENASE-1, CELLS, ASBESTOS, ACTIVATION, INDUCTION, LIPOPOLYSACCHARIDE, MECHANISMS, GENERATION",
author = "A. Haegens and Vernooy, {J. H. J.} and P. Heeringa and Mossman, {B. T.} and Wouters, {E. F. M.}",
year = "2008",
month = "2",
doi = "10.1183/09031936.00029307",
language = "English",
volume = "31",
pages = "252--260",
journal = "European Respiratory Journal",
issn = "0903-1936",
publisher = "EUROPEAN RESPIRATORY SOC JOURNALS LTD",
number = "2",

}

RIS

TY - JOUR

T1 - Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents

AU - Haegens, A.

AU - Vernooy, J. H. J.

AU - Heeringa, P.

AU - Mossman, B. T.

AU - Wouters, E. F. M.

PY - 2008/2

Y1 - 2008/2

N2 - During extensive inflammation, neutrophils undergo secondary necrosis causing myeloperoxidase (MPO) release that may damage resident lung cells. Recent observations suggest that MPO has pro-inflammatory properties, independent of its enzymatic activity. The aims of the present study were to characterise MPO internalisation by lung epithelial cells and to investigate the effect of MPO on oxidative stress, DNA damage and cytokine production by lung epithelial cells.Human alveolar and bronchial epithelial cells were stimulated with MPO, with or without priming the cells with pro-inflammatory stimuli. MPO protein was detected in the cell cytoplasm. Expression of haernoxygenase (HO)-1 and DNA strand breakage were determined. The production of interleukin (IL)-8 and -6 were measured.Analyses of MPO-stimulated cells demonstrated MPO presence in the cells. HO-1 expression was increased after MPO stimulation and increased further when cells were primed before MPO stimulation. MPO exposure also induced DNA strand breakage. Interestingly, MPO inhibited IL-8 production in bronchial, but not alveolar epithelium.In conclusion, alveolar and bronchial epithelial cells can internalise myeloperoxidase. Stimulation with myeloperoxidase increases haemoxygenase-1 expression and DNA strand breakage, suggesting cell damaging capacity of myeloperoxidase. In addition, myeloperoxidase inhibited interleukin-8 production by bronchial epithelial cells, indicating a negative feedback loop for neutrophil recruitment.

AB - During extensive inflammation, neutrophils undergo secondary necrosis causing myeloperoxidase (MPO) release that may damage resident lung cells. Recent observations suggest that MPO has pro-inflammatory properties, independent of its enzymatic activity. The aims of the present study were to characterise MPO internalisation by lung epithelial cells and to investigate the effect of MPO on oxidative stress, DNA damage and cytokine production by lung epithelial cells.Human alveolar and bronchial epithelial cells were stimulated with MPO, with or without priming the cells with pro-inflammatory stimuli. MPO protein was detected in the cell cytoplasm. Expression of haernoxygenase (HO)-1 and DNA strand breakage were determined. The production of interleukin (IL)-8 and -6 were measured.Analyses of MPO-stimulated cells demonstrated MPO presence in the cells. HO-1 expression was increased after MPO stimulation and increased further when cells were primed before MPO stimulation. MPO exposure also induced DNA strand breakage. Interestingly, MPO inhibited IL-8 production in bronchial, but not alveolar epithelium.In conclusion, alveolar and bronchial epithelial cells can internalise myeloperoxidase. Stimulation with myeloperoxidase increases haemoxygenase-1 expression and DNA strand breakage, suggesting cell damaging capacity of myeloperoxidase. In addition, myeloperoxidase inhibited interleukin-8 production by bronchial epithelial cells, indicating a negative feedback loop for neutrophil recruitment.

KW - inflammation

KW - myeloperoxidase

KW - neutrophilia

KW - oxidative stress

KW - NF-KAPPA-B

KW - GENE-EXPRESSION

KW - HEME OXYGENASE-1

KW - CELLS

KW - ASBESTOS

KW - ACTIVATION

KW - INDUCTION

KW - LIPOPOLYSACCHARIDE

KW - MECHANISMS

KW - GENERATION

U2 - 10.1183/09031936.00029307

DO - 10.1183/09031936.00029307

M3 - Article

VL - 31

SP - 252

EP - 260

JO - European Respiratory Journal

JF - European Respiratory Journal

SN - 0903-1936

IS - 2

ER -

ID: 4677182