Publication

Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells

Bayoumi, M., Bayley, H., Maglia, G. & Sapra, K. T., 3-Apr-2017, In : Scientific Reports. 7, p. 1-11 11 p., 45167.

Research output: Contribution to journalArticleAcademicpeer-review

Constructing a cell mimic is a major challenge posed by synthetic biologists. Efforts to this end have been primarily focused on lipid- and polymer-encapsulated containers, liposomes and polymersomes, respectively. Here, we introduce a multi-compartment, nested system comprising aqueous droplets stabilized in an oil/lipid mixture, all encapsulated in hydrogel. Functional capabilities (electrical and chemical communication) were imparted by protein nanopores spanning the lipid bilayer formed at the interface of the encapsulated aqueous droplets and the encasing hydrogel. Crucially, the compartmentalization enabled the formation of two adjoining lipid bilayers in a controlled manner, a requirement for the realization of a functional protocell or prototissue.

Original languageEnglish
Article number45167
Pages (from-to)1-11
Number of pages11
JournalScientific Reports
Volume7
Publication statusPublished - 3-Apr-2017

    Keywords

  • Journal Article, IN-VITRO RECONSTITUTION, INTERFACE BILAYERS, SELF-REPRODUCTION, RATIONAL DESIGN, GENE-EXPRESSION, VESICLES, PROTEIN, CHANNELS, BIOLOGY, SYSTEMS

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