Publication

Multicolor Electron Microscopy for Simultaneous Visualization of Multiple Molecular Species

Adams, S. R., Mackey, M. R., Ramachandra, R., Palida Lemieux, S. F., Steinbach, P., Bushong, E. A., Butko, M. T., Giepmans, B. N. G., Ellisman, M. H. & Tsien, R. Y., 17-Nov-2016, In : Cell Chemical Biology. 23, 11, p. 1417-1427 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Stephen R Adams
  • Mason R Mackey
  • Ranjan Ramachandra
  • Sakina F Palida Lemieux
  • Paul Steinbach
  • Eric A Bushong
  • Margaret T Butko
  • Ben N G Giepmans
  • Mark H Ellisman
  • Roger Y Tsien

Electron microscopy (EM) remains the primary method for imaging cellular and tissue ultrastructure, although simultaneous localization of multiple specific molecules continues to be a challenge for EM. We present a method for obtaining multicolor EM views of multiple subcellular components. The method uses sequential, localized deposition of different lanthanides by photosensitizers, small-molecule probes, or peroxidases. Detailed view of biological structures is created by overlaying conventional electron micrographs with pseudocolor lanthanide elemental maps derived from distinctive electron energy-loss spectra of each lanthanide deposit via energy-filtered transmission electron microscopy. This results in multicolor EM images analogous to multicolor fluorescence but with the benefit of the full spatial resolution of EM. We illustrate the power of this methodology by visualizing hippocampal astrocytes to show that processes from two astrocytes can share a single synapse. We also show that polyarginine-based cell-penetrating peptides enter the cell via endocytosis, and that newly synthesized PKMζ in cultured neurons preferentially localize to the postsynaptic membrane.

Original languageEnglish
Pages (from-to)1417-1427
Number of pages11
JournalCell Chemical Biology
Volume23
Issue number11
Publication statusPublished - 17-Nov-2016

    Keywords

  • CELL-PENETRATING PEPTIDES, FLUORESCENT CERAMIDE ANALOG, ASCORBATE PEROXIDASE, GOLGI-APPARATUS, IN-VIVO, LIGHT, PHOTOOXIDATION, ASTROCYTE, INHIBITION, MECHANISM

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