Mouse Lung Tissue Slice CultureWu, X., van Dijk, E. M., Bos, I. S. T., Kistemaker, L. E. M. & Gosens, R., 21-Feb-2019, Mouse cell culture. New York: Humana Press, p. 297-311 15 p. (Methods in Molecular Biology; vol. 1940).
Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
Precision-cut lung slices (PCLS) represent an ex vivo model widely used in visualizing interactions between lung structure and function. The major advantage of this technique is that the presence, differentiation state, and localization of the more than 40 cell types that make up the lung are in accordance with the physiological situation found in lung tissue, including the right localization and patterning of extracellular matrix elements. Here we describe the methodology involved in preparing and culturing PCLS followed by detailed practical information about their possible applications.
|Title of host publication||Mouse cell culture|
|Place of Publication||New York|
|Number of pages||15|
|Publication status||Published - 21-Feb-2019|
|Name||Methods in Molecular Biology|