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Monitoring of the deuterated and nondeuterated forms of levodopa and five metabolites in plasma and urine by LC-MS/MS

van de Merbel, N. C., Bronsema, K. J., Gorman, S. H. & Bakhtiar, R., Feb-2019, In : Bioanalysis. 11, 4, p. 279-294 16 p.

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  • Monitoring of the deuterated andnondeuterated forms of levodopa and fivemetabolites in plasma and urine byLC–MS/MS

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DOI

To compare pharmacokinetics, metabolism and excretion of levodopa and a triply deuterated form, which is being developed as an improved treatment for Parkinson's disease, methods were needed for quantification of the deuterated and nondeuterated forms of levodopa and five metabolites in human plasma and urine. Results: The natural heavy isotopes in the nondeuterated compounds caused an absolute contribution of up to 100% in the response of the deuterated compounds. Similarly, heavy isotopes in the deuterated analytes contributed to the response of the internal standards, but this did not affect the reliability of the results. Conclusion: Deuterated and nondeuterated analytes can be quantified together by LC-MS/MS, but overestimation of the concentrations of the deuterated molecules may be unavoidable and a careful interpretation of the concentration data is essential.

Original languageEnglish
Pages (from-to)279-294
Number of pages16
JournalBioanalysis
Volume11
Issue number4
Publication statusPublished - Feb-2019

    Keywords

  • deuterated drugs, interference, LC-MS, MS, levodopa, natural heavy isotopes, QUANTITATIVE-DETERMINATION, PARKINSONS-DISEASE, L-DOPA, DEUTERIUM, DERIVATIZATION, QUANTIFICATION, DISCOVERY, CARBIDOPA, DRUG

ID: 103506385