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Molecular phylogeny of the Chironomus genus deduced from nucleotide sequences of two nuclear genes, ssp160 and the Globin 2b gene

Makarevich, I. F., Berezikov, E. V., Guryev, V. P. & Blinov, A. G., Jul-2000, In : Molecular biology. 34, 4, p. 606-612 7 p.

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  • Molecular phylogeny of theChironomus genus deduced from nucleotide sequences of two nuclear genes,ssp 160 and the globin 2b gene

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DOI

Two genes were employed to study phylogenetic relatedness of the Chironomus species: the protein-coding, salivary gland-specific ssp160 gene, and the globin 2b (gb2b) gene. By using PCR, it was demonstrated that all the 38 Chironomus species analyzed possess the gb2b gene, while only 13 have the ssp160 gene. Partial nucleotide sequences of the genes of 22 species were determined. The data obtained were employed to construct phylogenetic trees which appeared to be topologically similar and revealed five groups of phylogenetically closely related species. Combining the data obtained in the studies of nuclear and mitochondrial genes, a molecular-data-based scenario could be suggested for the Chironomus genus evolution.

Original languageEnglish
Pages (from-to)606-612
Number of pages7
JournalMolecular biology
Volume34
Issue number4
Publication statusPublished - Jul-2000

    Keywords

  • 2b globin gene, Chironomids (Chironomidae, Chironomus), Evolution of nuclear and mitochondrial genes, Phylogeny, ssp160 gene

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