Publication

Molecular epidemiology of Shigella flexneri isolated from pediatrics in a diarrhea-endemic area of Khyber Pakhtunkhwa, Pakistan

Nisa, I., Qasim, M., Driessen, A., Nijland, J., Bari, F., Haroon, M., Rahman, H., Yasin, N., Khan, T. A., Hussain, M. & Hussain, W., May-2020, In : European Journal of Clinical Microbiology & Infectious Diseases. 39, 5, p. 971-985 15 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Nisa, I., Qasim, M., Driessen, A., Nijland, J., Bari, F., Haroon, M., ... Hussain, W. (2020). Molecular epidemiology of Shigella flexneri isolated from pediatrics in a diarrhea-endemic area of Khyber Pakhtunkhwa, Pakistan. European Journal of Clinical Microbiology & Infectious Diseases, 39(5), 971-985. https://doi.org/10.1007/s10096-020-03811-0

Author

Nisa, Iqbal ; Qasim, Muhammad ; Driessen, Arnold ; Nijland, Jeroen ; Bari, Fazli ; Haroon, Mohammad ; Rahman, Hazir ; Yasin, Nusrat ; Khan, Taj Ali ; Hussain, Mubbashir ; Hussain, Waheed. / Molecular epidemiology of Shigella flexneri isolated from pediatrics in a diarrhea-endemic area of Khyber Pakhtunkhwa, Pakistan. In: European Journal of Clinical Microbiology & Infectious Diseases. 2020 ; Vol. 39, No. 5. pp. 971-985.

Harvard

Nisa, I, Qasim, M, Driessen, A, Nijland, J, Bari, F, Haroon, M, Rahman, H, Yasin, N, Khan, TA, Hussain, M & Hussain, W 2020, 'Molecular epidemiology of Shigella flexneri isolated from pediatrics in a diarrhea-endemic area of Khyber Pakhtunkhwa, Pakistan', European Journal of Clinical Microbiology & Infectious Diseases, vol. 39, no. 5, pp. 971-985. https://doi.org/10.1007/s10096-020-03811-0

Standard

Molecular epidemiology of Shigella flexneri isolated from pediatrics in a diarrhea-endemic area of Khyber Pakhtunkhwa, Pakistan. / Nisa, Iqbal; Qasim, Muhammad; Driessen, Arnold; Nijland, Jeroen; Bari, Fazli ; Haroon, Mohammad ; Rahman, Hazir ; Yasin, Nusrat ; Khan, Taj Ali ; Hussain, Mubbashir ; Hussain, Waheed.

In: European Journal of Clinical Microbiology & Infectious Diseases, Vol. 39, No. 5, 05.2020, p. 971-985.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Nisa I, Qasim M, Driessen A, Nijland J, Bari F, Haroon M et al. Molecular epidemiology of Shigella flexneri isolated from pediatrics in a diarrhea-endemic area of Khyber Pakhtunkhwa, Pakistan. European Journal of Clinical Microbiology & Infectious Diseases. 2020 May;39(5):971-985. https://doi.org/10.1007/s10096-020-03811-0


BibTeX

@article{bf4c677a2dbb47d1bd6136d0a267a216,
title = "Molecular epidemiology of Shigella flexneri isolated from pediatrics in a diarrhea-endemic area of Khyber Pakhtunkhwa, Pakistan",
abstract = "Shigella flexneri is considered as an important causative agent of Shigellosis causing diarrhea in the countries with a low socioeconomic status. No study has been carried out on the molecular prevalence of S. flexneri in Khyber Pakhtunkhwa, Pakistan. So this study was designed to evaluate the molecular prevalence of S. flexneri and their associated risk factors. A total of 2014 diarrheal stool samples were collected from January 2016 to May 2017 from pediatrics patients of Khyber Pakhtunkhwa followed by identification of S. flexneri through biochemical, serological, and molecular methods. The overall prevalence of Shigella species was found to be 7.9{\%} (n = 160). The predominant Shigella specie was S. flexneri (n = 155, 96.8{\%}) followed by S. boydii (n = 5, 3.1{\%}). Interestingly, no sample was found positive for S. sonnei and S. dysenteriae. The majority of Shigellosis cases occurred from June to September. Potential risk factors related with Shigellosis were unhygienic latrine usage, bad hand washing, and consumption of unhygienic food and water, and pipe leakage in the sewage system. In this study, we have observed a high number of Shigellosis cases especially those caused by S. flexneri. It is suggested that effective health awareness programs should be organized by the regional health authorities to minimize the magnitude of pediatrics Shigellosis.",
keywords = "S. flexneri, Pediatrics, Molecular epidemiology, Diarrhea, Endemic area, Pakistan",
author = "Iqbal Nisa and Muhammad Qasim and Arnold Driessen and Jeroen Nijland and Fazli Bari and Mohammad Haroon and Hazir Rahman and Nusrat Yasin and Khan, {Taj Ali} and Mubbashir Hussain and Waheed Hussain",
year = "2020",
month = "5",
doi = "10.1007/s10096-020-03811-0",
language = "English",
volume = "39",
pages = "971--985",
journal = "European Journal of Clinical Microbiology & Infectious Diseases",
issn = "0934-9723",
publisher = "SPRINGER",
number = "5",

}

RIS

TY - JOUR

T1 - Molecular epidemiology of Shigella flexneri isolated from pediatrics in a diarrhea-endemic area of Khyber Pakhtunkhwa, Pakistan

AU - Nisa, Iqbal

AU - Qasim, Muhammad

AU - Driessen, Arnold

AU - Nijland, Jeroen

AU - Bari, Fazli

AU - Haroon, Mohammad

AU - Rahman, Hazir

AU - Yasin, Nusrat

AU - Khan, Taj Ali

AU - Hussain, Mubbashir

AU - Hussain, Waheed

PY - 2020/5

Y1 - 2020/5

N2 - Shigella flexneri is considered as an important causative agent of Shigellosis causing diarrhea in the countries with a low socioeconomic status. No study has been carried out on the molecular prevalence of S. flexneri in Khyber Pakhtunkhwa, Pakistan. So this study was designed to evaluate the molecular prevalence of S. flexneri and their associated risk factors. A total of 2014 diarrheal stool samples were collected from January 2016 to May 2017 from pediatrics patients of Khyber Pakhtunkhwa followed by identification of S. flexneri through biochemical, serological, and molecular methods. The overall prevalence of Shigella species was found to be 7.9% (n = 160). The predominant Shigella specie was S. flexneri (n = 155, 96.8%) followed by S. boydii (n = 5, 3.1%). Interestingly, no sample was found positive for S. sonnei and S. dysenteriae. The majority of Shigellosis cases occurred from June to September. Potential risk factors related with Shigellosis were unhygienic latrine usage, bad hand washing, and consumption of unhygienic food and water, and pipe leakage in the sewage system. In this study, we have observed a high number of Shigellosis cases especially those caused by S. flexneri. It is suggested that effective health awareness programs should be organized by the regional health authorities to minimize the magnitude of pediatrics Shigellosis.

AB - Shigella flexneri is considered as an important causative agent of Shigellosis causing diarrhea in the countries with a low socioeconomic status. No study has been carried out on the molecular prevalence of S. flexneri in Khyber Pakhtunkhwa, Pakistan. So this study was designed to evaluate the molecular prevalence of S. flexneri and their associated risk factors. A total of 2014 diarrheal stool samples were collected from January 2016 to May 2017 from pediatrics patients of Khyber Pakhtunkhwa followed by identification of S. flexneri through biochemical, serological, and molecular methods. The overall prevalence of Shigella species was found to be 7.9% (n = 160). The predominant Shigella specie was S. flexneri (n = 155, 96.8%) followed by S. boydii (n = 5, 3.1%). Interestingly, no sample was found positive for S. sonnei and S. dysenteriae. The majority of Shigellosis cases occurred from June to September. Potential risk factors related with Shigellosis were unhygienic latrine usage, bad hand washing, and consumption of unhygienic food and water, and pipe leakage in the sewage system. In this study, we have observed a high number of Shigellosis cases especially those caused by S. flexneri. It is suggested that effective health awareness programs should be organized by the regional health authorities to minimize the magnitude of pediatrics Shigellosis.

KW - S. flexneri

KW - Pediatrics

KW - Molecular epidemiology

KW - Diarrhea

KW - Endemic area

KW - Pakistan

U2 - 10.1007/s10096-020-03811-0

DO - 10.1007/s10096-020-03811-0

M3 - Article

VL - 39

SP - 971

EP - 985

JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

SN - 0934-9723

IS - 5

ER -

ID: 117374346