Publication

Molecular cloning and characterisation of the ribC gene from Bacillus subtilis: A point mutation in ribC results in riboflavin overproduction

Coquard, D., Huecas, M., Ott, M., vanDijl, JM., VanLoon, APGM. & Hohmann, HP., 18-Mar-1997, In : Molecular & General Genetics. 254, 1, p. 81-84 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • D Coquard
  • M Huecas
  • M Ott
  • JM vanDijl
  • APGM VanLoon
  • HP Hohmann

A mutation leading to roseoflavin resistance and deregulated riboflavin biosynthesis was mapped in the genome of the riboflavin-overproducing Bacillus subtilis strains RB52 and RB50 at map position 147 degrees. The chromosomal location indicates that the deregulating mutation in RB52 and RB50 is an allele of the previously identified ribC mutation. We cloned the ribC gene and found that it encodes a putative 36-kDa protein. Surprisingly, RibC has significant sequence similarity to flavin kinases and FAD synthases from various other bacterial species. By comparing the deduced amino acid sequence of RibC from the wild-type parent strain of RB50 with the RibC sequence from the riboflavin-overexpressing RB50 mutant we identified a point mutation that resulted in a Gly to Ser exchange in the C-terminal region of the product.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalMolecular & General Genetics
Volume254
Issue number1
Publication statusPublished - 18-Mar-1997

    Keywords

  • Bacillus subtilis, riboflavin biosynthesis, ribC, flavin kinase, FAD synthase, ESCHERICHIA-COLI, NUCLEOTIDE-SEQUENCE, POLYNUCLEOTIDE PHOSPHORYLASE, BIOSYNTHESIS GENES, OPERON, ORGANIZATION, GENOME, INFB

ID: 6506942