Publication

Molecular analysis of the UV-inducible pili operon from Sulfolobus acidocaldarius

Wolferen, M. V., Ajon, M., Driessen, A. J. M. & Albers, S-V., 2013, In : MicrobiologyOpen. 2, 6, p. 928-937 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

Upon ultraviolet (UV) stress, hyperthermophilic Sulfolobus species show a highly induced transcription of a gene cluster responsible for pili biogenesis: the UV-inducible pili operon (ups operon). This operon is involved in UV-induced pili assembly, cellular aggregation, and subsequent DNA exchange between cells. As the system increases the fitness of Sulfolobus cells after UV light exposure, we assume that transfer of DNA takes place in order to repair UV-induced DNA damages via homologous recombination. Here, we studied all genes present in the ups cluster via gene deletion analysis with a focus on UpsX, a protein that shows no identifiable functional domains. UspX does not seem to be structurally essential for UV-induced pili formation and cellular aggregation, but appears to be important for efficient DNA transfer. In addition, we could show that pilin subunits UpsA and UpsB probably both function as major pilin subunits in the ups pili.
Original languageEnglish
Pages (from-to)928-937
Number of pages10
JournalMicrobiologyOpen
Volume2
Issue number6
Publication statusPublished - 2013

    Keywords

  • type IV pili, DNA exchange, conjugation, archaea
Related Publications
  1. Corrigendum: Molecular analysis of the UV-inducible pili operon from Sulfolobus acidocaldarius (vol 2, pg 928, 2013)

    van Wolferen, M., Ajon, M., Driessen, A. J. M. & Albers, S-V., Dec-2016, In : MicrobiologyOpen. 5, 6, p. 1085-1085 1 p.

    Research output: Contribution to journalArticleAcademicpeer-review

View all (1) »

Download statistics

No data available

ID: 2394895