Publication

Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non-Digestible Carbohydrates

Kong, C., Elderman, M., Cheng, L., de Haan, B. J., Nauta, A. & de Vos, P., Sep-2019, In : Molecular Nutrition & Food Research. 63, 17, 10 p., 1900303.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Kong, C., Elderman, M., Cheng, L., de Haan, B. J., Nauta, A., & de Vos, P. (2019). Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non-Digestible Carbohydrates. Molecular Nutrition & Food Research, 63(17), [1900303]. https://doi.org/10.1002/mnfr.201900303

Author

Kong, Chunli ; Elderman, Marlies ; Cheng, Lianghui ; de Haan, Bart J ; Nauta, Arjen ; de Vos, Paul. / Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non-Digestible Carbohydrates. In: Molecular Nutrition & Food Research. 2019 ; Vol. 63, No. 17.

Harvard

Kong, C, Elderman, M, Cheng, L, de Haan, BJ, Nauta, A & de Vos, P 2019, 'Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non-Digestible Carbohydrates', Molecular Nutrition & Food Research, vol. 63, no. 17, 1900303. https://doi.org/10.1002/mnfr.201900303

Standard

Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non-Digestible Carbohydrates. / Kong, Chunli; Elderman, Marlies; Cheng, Lianghui; de Haan, Bart J; Nauta, Arjen; de Vos, Paul.

In: Molecular Nutrition & Food Research, Vol. 63, No. 17, 1900303, 09.2019.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Kong C, Elderman M, Cheng L, de Haan BJ, Nauta A, de Vos P. Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non-Digestible Carbohydrates. Molecular Nutrition & Food Research. 2019 Sep;63(17). 1900303. https://doi.org/10.1002/mnfr.201900303


BibTeX

@article{a8261b0ab581485fb93526c28e524535,
title = "Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non-Digestible Carbohydrates",
abstract = "Scope The epithelial glycocalyx development is of great importance for microbial colonization. Human milk oligosaccharides (hMOs) and non-digestible carbohydrates (NDCs) may modulate glycocalyx development. Methods and results The effects of hMOs and NDCs on human gut epithelial cells (Caco2) are investigated by quantifying thickness and area coverage of adsorbed albumin, heparan sulfate (HS), and hyaluronic acid (HA) in the glycocalyx. Effects of hMOs (2 '-FL and 3-FL) and NDCs [inulins with degrees of polymerization (DP) (DP3-DP10, DP10-DP60, DP30-DP60) and pectins with degrees of methylation (DM) (DM7, DM55, DM69)] are tested using immunofluorescence staining at 1 and 5 days stimulation. HMOs show a significant enhancing effect on glycocalyx development but effects are structure-dependent. 3-FL induces a stronger albumin adsorption and increases HS and HA stronger than 2 '-FL. The DP3-DP10, DP30-60 inulins also increase glycocalyx development in a structure-dependent manner as DP3-DP10 selectively increases HS, while DP30-DP60 specifically increases HA. Pectins have less effects, and only increase albumin adsorption. Conclusion Here, it is shown that 2 '-FL and 3-FL and inulins stimulate glycocalyx development in a structure-dependent fashion. This may contribute to formulation of effective hMO and NDC formulations in infant formulas to support microbial colonization and gut barrier function.",
keywords = "glycocalyx, gut barrier function, human milk oligosaccharides, intestinal epithelium, non-digestible carbohydrates, ENDOTHELIAL GLYCOCALYX, IN-VIVO, PERMEABILITY, ALBUMIN",
author = "Chunli Kong and Marlies Elderman and Lianghui Cheng and {de Haan}, {Bart J} and Arjen Nauta and {de Vos}, Paul",
note = "This article is protected by copyright. All rights reserved.",
year = "2019",
month = "9",
doi = "10.1002/mnfr.201900303",
language = "English",
volume = "63",
journal = "Molecular Nutrition & Food Research",
issn = "1613-4125",
publisher = "WILEY",
number = "17",

}

RIS

TY - JOUR

T1 - Modulation of Intestinal Epithelial Glycocalyx Development by Human Milk Oligosaccharides and Non-Digestible Carbohydrates

AU - Kong, Chunli

AU - Elderman, Marlies

AU - Cheng, Lianghui

AU - de Haan, Bart J

AU - Nauta, Arjen

AU - de Vos, Paul

N1 - This article is protected by copyright. All rights reserved.

PY - 2019/9

Y1 - 2019/9

N2 - Scope The epithelial glycocalyx development is of great importance for microbial colonization. Human milk oligosaccharides (hMOs) and non-digestible carbohydrates (NDCs) may modulate glycocalyx development. Methods and results The effects of hMOs and NDCs on human gut epithelial cells (Caco2) are investigated by quantifying thickness and area coverage of adsorbed albumin, heparan sulfate (HS), and hyaluronic acid (HA) in the glycocalyx. Effects of hMOs (2 '-FL and 3-FL) and NDCs [inulins with degrees of polymerization (DP) (DP3-DP10, DP10-DP60, DP30-DP60) and pectins with degrees of methylation (DM) (DM7, DM55, DM69)] are tested using immunofluorescence staining at 1 and 5 days stimulation. HMOs show a significant enhancing effect on glycocalyx development but effects are structure-dependent. 3-FL induces a stronger albumin adsorption and increases HS and HA stronger than 2 '-FL. The DP3-DP10, DP30-60 inulins also increase glycocalyx development in a structure-dependent manner as DP3-DP10 selectively increases HS, while DP30-DP60 specifically increases HA. Pectins have less effects, and only increase albumin adsorption. Conclusion Here, it is shown that 2 '-FL and 3-FL and inulins stimulate glycocalyx development in a structure-dependent fashion. This may contribute to formulation of effective hMO and NDC formulations in infant formulas to support microbial colonization and gut barrier function.

AB - Scope The epithelial glycocalyx development is of great importance for microbial colonization. Human milk oligosaccharides (hMOs) and non-digestible carbohydrates (NDCs) may modulate glycocalyx development. Methods and results The effects of hMOs and NDCs on human gut epithelial cells (Caco2) are investigated by quantifying thickness and area coverage of adsorbed albumin, heparan sulfate (HS), and hyaluronic acid (HA) in the glycocalyx. Effects of hMOs (2 '-FL and 3-FL) and NDCs [inulins with degrees of polymerization (DP) (DP3-DP10, DP10-DP60, DP30-DP60) and pectins with degrees of methylation (DM) (DM7, DM55, DM69)] are tested using immunofluorescence staining at 1 and 5 days stimulation. HMOs show a significant enhancing effect on glycocalyx development but effects are structure-dependent. 3-FL induces a stronger albumin adsorption and increases HS and HA stronger than 2 '-FL. The DP3-DP10, DP30-60 inulins also increase glycocalyx development in a structure-dependent manner as DP3-DP10 selectively increases HS, while DP30-DP60 specifically increases HA. Pectins have less effects, and only increase albumin adsorption. Conclusion Here, it is shown that 2 '-FL and 3-FL and inulins stimulate glycocalyx development in a structure-dependent fashion. This may contribute to formulation of effective hMO and NDC formulations in infant formulas to support microbial colonization and gut barrier function.

KW - glycocalyx

KW - gut barrier function

KW - human milk oligosaccharides

KW - intestinal epithelium

KW - non-digestible carbohydrates

KW - ENDOTHELIAL GLYCOCALYX

KW - IN-VIVO

KW - PERMEABILITY

KW - ALBUMIN

U2 - 10.1002/mnfr.201900303

DO - 10.1002/mnfr.201900303

M3 - Article

VL - 63

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 17

M1 - 1900303

ER -

ID: 84007048