Publication

Mineralization of the herbicide 2,3,6-trichlorobenzoic acid by a co-culture of anaerobic and aerobic bacteria

Gerritse, J. & Gottschal, J. C., 1-Jul-1992, In : FEMS Microbial Ecology. 101, 2, p. 89-98 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Jan Gerritse
  • Jan C. Gottschal

Bacteria from an anaerobic enrichment reductively removed chlorine from the ortho- position of 2,3,6-trichlorobenzoic acid (2,3,6-TBA) producing 2,5-dichlorobenzoate (2,5-DBA). The strictly aerobic bacterium Pseudomonas aeruginosa JB2 subsequently used 2,5-DBA as a growth substrate in the presence of oxygen. The anaerobic dechlorinating microbial population was grown with P. aeruginosa JB2 in continuous culture. Inside the liquid culture, a nylon netting, on a stainless-steel support, contained vermiculite particles to provide a strictly anaerobic environment within the aerated culture. Complete mineralization of 2,3,6-TBA depended on the extent of oxygen input into the reactor. Under strictly anaerobic conditions 2,5-DBA and Cl- were produced stoichiometrically through the reductive dechlorination of 2,3,6-TBA. This process of reductive dechlorination was not inhibited by (moderate) aeration resulting in an O2-concentration of 0.3-0.5-mu-M in the culture liquid.

Original languageEnglish
Pages (from-to)89-98
Number of pages10
JournalFEMS Microbial Ecology
Volume101
Issue number2
Publication statusPublished - 1-Jul-1992

    Keywords

  • COCULTURE, ANAEROBIC PLUS AEROBIC BACTERIA, 2,3,6-TRICHLOROBENZOIC ACID, REDUCTIVE DECHLORINATION, 2,5-DICHLOROBENZOIC ACID, MICROAEROBIC MINERALIZATION, HALOGENATED AROMATIC-COMPOUNDS, ALCALIGENES-DENITRIFICANS, PSEUDOMONAS-AERUGINOSA, METHANOGENIC BACTERIA, DEGRADATION, DEHALOGENATION, SEDIMENT, ISOMERS, SOIL

ID: 6320850