Publication

microRNAs associated with the different human Argonaute proteins

Dueck, A., Ziegler, C., Eichner, A., Berezikov, E. & Meister, G., Oct-2012, In : Nucleic Acids Research. 40, 19, p. 9850-9862 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Dueck, A., Ziegler, C., Eichner, A., Berezikov, E., & Meister, G. (2012). microRNAs associated with the different human Argonaute proteins. Nucleic Acids Research, 40(19), 9850-9862. https://doi.org/10.1093/nar/gks705

Author

Dueck, Anne ; Ziegler, Christian ; Eichner, Alexander ; Berezikov, Eugene ; Meister, Gunter. / microRNAs associated with the different human Argonaute proteins. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 19. pp. 9850-9862.

Harvard

Dueck, A, Ziegler, C, Eichner, A, Berezikov, E & Meister, G 2012, 'microRNAs associated with the different human Argonaute proteins', Nucleic Acids Research, vol. 40, no. 19, pp. 9850-9862. https://doi.org/10.1093/nar/gks705

Standard

microRNAs associated with the different human Argonaute proteins. / Dueck, Anne; Ziegler, Christian; Eichner, Alexander; Berezikov, Eugene; Meister, Gunter.

In: Nucleic Acids Research, Vol. 40, No. 19, 10.2012, p. 9850-9862.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Dueck A, Ziegler C, Eichner A, Berezikov E, Meister G. microRNAs associated with the different human Argonaute proteins. Nucleic Acids Research. 2012 Oct;40(19):9850-9862. https://doi.org/10.1093/nar/gks705


BibTeX

@article{b356c14402c843f59cc79f7ac81bd0b3,
title = "microRNAs associated with the different human Argonaute proteins",
abstract = "MicroRNAs (miRNAs) are small noncoding RNAs that function in literally all cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them to specific target sites located in the 3'-untranslated region (3'-UTR) of target mRNAs leading to translational repression and deadenylation-induced mRNA degradation. Most miRNAs are processed from hairpin-structured precursors by the consecutive action of the RNase III enzymes Drosha and Dicer. However, processing of miR-451 is Dicer independent and cleavage is mediated by the endonuclease Ago2. Here we have characterized miR-451 sequence and structure requirements for processing as well as sorting of miRNAs into different Ago proteins. Pre-miR-451 appears to be optimized for Ago2 cleavage and changes result in reduced processing. In addition, we show that the mature miR-451 only associates with Ago2 suggesting that mature miRNAs are not exchanged between different members of the Ago protein family. Based on cloning and deep sequencing of endogenous miRNAs associated with Ago1-3, we do not find evidence for miRNA sorting in human cells. However, Ago identity appears to influence the length of some miRNAs, while others remain unaffected.",
keywords = "MESSENGER-RNA TRANSLATION, PASSENGER-STRAND, SIRNA, IDENTIFICATION, BIOGENESIS, MECHANISMS, DROSOPHILA, COMPLEXES, CLEAVAGE, CELLS",
author = "Anne Dueck and Christian Ziegler and Alexander Eichner and Eugene Berezikov and Gunter Meister",
year = "2012",
month = oct,
doi = "10.1093/nar/gks705",
language = "English",
volume = "40",
pages = "9850--9862",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "19",

}

RIS

TY - JOUR

T1 - microRNAs associated with the different human Argonaute proteins

AU - Dueck, Anne

AU - Ziegler, Christian

AU - Eichner, Alexander

AU - Berezikov, Eugene

AU - Meister, Gunter

PY - 2012/10

Y1 - 2012/10

N2 - MicroRNAs (miRNAs) are small noncoding RNAs that function in literally all cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them to specific target sites located in the 3'-untranslated region (3'-UTR) of target mRNAs leading to translational repression and deadenylation-induced mRNA degradation. Most miRNAs are processed from hairpin-structured precursors by the consecutive action of the RNase III enzymes Drosha and Dicer. However, processing of miR-451 is Dicer independent and cleavage is mediated by the endonuclease Ago2. Here we have characterized miR-451 sequence and structure requirements for processing as well as sorting of miRNAs into different Ago proteins. Pre-miR-451 appears to be optimized for Ago2 cleavage and changes result in reduced processing. In addition, we show that the mature miR-451 only associates with Ago2 suggesting that mature miRNAs are not exchanged between different members of the Ago protein family. Based on cloning and deep sequencing of endogenous miRNAs associated with Ago1-3, we do not find evidence for miRNA sorting in human cells. However, Ago identity appears to influence the length of some miRNAs, while others remain unaffected.

AB - MicroRNAs (miRNAs) are small noncoding RNAs that function in literally all cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them to specific target sites located in the 3'-untranslated region (3'-UTR) of target mRNAs leading to translational repression and deadenylation-induced mRNA degradation. Most miRNAs are processed from hairpin-structured precursors by the consecutive action of the RNase III enzymes Drosha and Dicer. However, processing of miR-451 is Dicer independent and cleavage is mediated by the endonuclease Ago2. Here we have characterized miR-451 sequence and structure requirements for processing as well as sorting of miRNAs into different Ago proteins. Pre-miR-451 appears to be optimized for Ago2 cleavage and changes result in reduced processing. In addition, we show that the mature miR-451 only associates with Ago2 suggesting that mature miRNAs are not exchanged between different members of the Ago protein family. Based on cloning and deep sequencing of endogenous miRNAs associated with Ago1-3, we do not find evidence for miRNA sorting in human cells. However, Ago identity appears to influence the length of some miRNAs, while others remain unaffected.

KW - MESSENGER-RNA TRANSLATION

KW - PASSENGER-STRAND

KW - SIRNA

KW - IDENTIFICATION

KW - BIOGENESIS

KW - MECHANISMS

KW - DROSOPHILA

KW - COMPLEXES

KW - CLEAVAGE

KW - CELLS

U2 - 10.1093/nar/gks705

DO - 10.1093/nar/gks705

M3 - Article

C2 - 22844086

VL - 40

SP - 9850

EP - 9862

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 19

ER -

ID: 5706976