Microglia replenished OHSC: A culture system to study in vivo like adult microgliaMasuch, A., van der Pijl, R., Füner, L., Wolf, Y., Eggen, B., Boddeke, E. & Biber, K., Aug-2016, In : Glia. 64, 8, p. 1285-1297 13 p.
Research output: Contribution to journal › Article › Academic › peer-review
Recent data suggest that ramified microglia fulfil various tasks in the brain. However, to investigate this unique cell type cultured primary microglia are only a poor model. We here describe a method to deplete and repopulate organotypic hippocampal slice cultures (OHSC) with ramified microglia isolated from adult mouse brain creating microglia-replenished OHSC (Mrep-OHSC). Replenished microglia integrate into the tissue and ramify to a degree indistinguishable from their counterparts in the mouse brain. Moreover, wild-type slices replenished with microglia from TNFα-deficient animals provide similar results as OHSC prepared from microglia-specific TNFα-knockout mice (CX3CR1(cre) /TNFα(fl/fl) ). Furthermore, this study demonstrates that replenished microglia in OHSC maintain original functions and properties acquired in vivo. Microglia from ERCC1(Δ/ko) mice, a mouse model of accelerated aging, maintain enhanced Mac2 expression and their activated phenotype after replenishment to wild-type OHSC tissue. Thus, the present study demonstrates that Mrep-OHSC are a unique tool to construct chimeric brain slices allowing studying the function of different phenotypes of in vivo like microglia in a tissue culture setting. GLIA 2016.
|Number of pages||13|
|Publication status||Published - Aug-2016|
- hippocampus, NMDA, neuroprotection, TNF alpha, priming, ERCC1, HIPPOCAMPAL SLICE CULTURES, CENTRAL-NERVOUS-SYSTEM, TUMOR-NECROSIS-FACTOR, DNA-REPAIR, BRAIN, CELLS, INJURY, MICE, EXCITOTOXICITY, NEURONS