Publication

Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota

van Veelen, H. P. J., Salles, J. F. & Tieleman, B. I., May-2018, In : The ISME journal. 12, 5, p. 1375-1388 14 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

van Veelen, H. P. J., Salles, J. F., & Tieleman, B. I. (2018). Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota. The ISME journal, 12(5), 1375-1388. https://doi.org/10.1038/s41396-018-0067-3

Author

van Veelen, H Pieter J ; Salles, Joana Falcão ; Tieleman, B Irene. / Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota. In: The ISME journal. 2018 ; Vol. 12, No. 5. pp. 1375-1388.

Harvard

van Veelen, HPJ, Salles, JF & Tieleman, BI 2018, 'Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota', The ISME journal, vol. 12, no. 5, pp. 1375-1388. https://doi.org/10.1038/s41396-018-0067-3

Standard

Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota. / van Veelen, H Pieter J; Salles, Joana Falcão; Tieleman, B Irene.

In: The ISME journal, Vol. 12, No. 5, 05.2018, p. 1375-1388.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

van Veelen HPJ, Salles JF, Tieleman BI. Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota. The ISME journal. 2018 May;12(5):1375-1388. https://doi.org/10.1038/s41396-018-0067-3


BibTeX

@article{9fd9c05c3ee04a3ea9f4f021621a1374,
title = "Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota",
abstract = "The microbiome is essential for development, health and homeostasis throughout an animal's life. Yet, the origins and transmission processes governing animal microbiomes remain elusive for non-human vertebrates, oviparous vertebrates in particular. Eggs may function as transgenerational carriers of the maternal microbiome, warranting characterisation of egg microbiome assembly. Here, we investigated maternal and environmental contributions to avian eggshell microbiota in wild passerine birds: woodlark Lullula arborea and skylark Alauda arvensis. Using 16S rRNA gene sequencing, we demonstrated in both lark species, at the population and within-nest levels, that bacterial communities of freshly laid eggs were distinct from the female cloacal microbiome. Instead, soil-borne bacteria appeared to thrive on freshly laid eggs, and eggshell microbiota composition strongly resembled maternal skin, body feather and nest material communities, sources in direct contact with laid eggs. Finally, phylogenetic structure analysis and microbial source tracking underscored species sorting from directly contacting sources rather than in vivo-transferred symbionts. The female-egg-nest system allowed an integrative assessment of avian egg microbiome assembly, revealing mixed modes of symbiont acquisition not previously documented for vertebrate eggs. Our findings illuminated egg microbiome origins, which suggested a limited potential of eggshells for transgenerational transmission, encouraging further investigation of eggshell microbiome functions in vertebrates.",
keywords = "TRANS-SHELL INFECTION, GUT MICROBIOTA, EGG VIABILITY, BACTERIAL, INCUBATION, LIFE, TRANSMISSION, COMMUNITIES, DIVERSITY, EVOLUTION",
author = "{van Veelen}, {H Pieter J} and Salles, {Joana Falc{\~a}o} and Tieleman, {B Irene}",
year = "2018",
month = "5",
doi = "10.1038/s41396-018-0067-3",
language = "English",
volume = "12",
pages = "1375--1388",
journal = "ISME Journal",
issn = "1751-7370",
publisher = "Nature Publishing Group",
number = "5",

}

RIS

TY - JOUR

T1 - Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota

AU - van Veelen, H Pieter J

AU - Salles, Joana Falcão

AU - Tieleman, B Irene

PY - 2018/5

Y1 - 2018/5

N2 - The microbiome is essential for development, health and homeostasis throughout an animal's life. Yet, the origins and transmission processes governing animal microbiomes remain elusive for non-human vertebrates, oviparous vertebrates in particular. Eggs may function as transgenerational carriers of the maternal microbiome, warranting characterisation of egg microbiome assembly. Here, we investigated maternal and environmental contributions to avian eggshell microbiota in wild passerine birds: woodlark Lullula arborea and skylark Alauda arvensis. Using 16S rRNA gene sequencing, we demonstrated in both lark species, at the population and within-nest levels, that bacterial communities of freshly laid eggs were distinct from the female cloacal microbiome. Instead, soil-borne bacteria appeared to thrive on freshly laid eggs, and eggshell microbiota composition strongly resembled maternal skin, body feather and nest material communities, sources in direct contact with laid eggs. Finally, phylogenetic structure analysis and microbial source tracking underscored species sorting from directly contacting sources rather than in vivo-transferred symbionts. The female-egg-nest system allowed an integrative assessment of avian egg microbiome assembly, revealing mixed modes of symbiont acquisition not previously documented for vertebrate eggs. Our findings illuminated egg microbiome origins, which suggested a limited potential of eggshells for transgenerational transmission, encouraging further investigation of eggshell microbiome functions in vertebrates.

AB - The microbiome is essential for development, health and homeostasis throughout an animal's life. Yet, the origins and transmission processes governing animal microbiomes remain elusive for non-human vertebrates, oviparous vertebrates in particular. Eggs may function as transgenerational carriers of the maternal microbiome, warranting characterisation of egg microbiome assembly. Here, we investigated maternal and environmental contributions to avian eggshell microbiota in wild passerine birds: woodlark Lullula arborea and skylark Alauda arvensis. Using 16S rRNA gene sequencing, we demonstrated in both lark species, at the population and within-nest levels, that bacterial communities of freshly laid eggs were distinct from the female cloacal microbiome. Instead, soil-borne bacteria appeared to thrive on freshly laid eggs, and eggshell microbiota composition strongly resembled maternal skin, body feather and nest material communities, sources in direct contact with laid eggs. Finally, phylogenetic structure analysis and microbial source tracking underscored species sorting from directly contacting sources rather than in vivo-transferred symbionts. The female-egg-nest system allowed an integrative assessment of avian egg microbiome assembly, revealing mixed modes of symbiont acquisition not previously documented for vertebrate eggs. Our findings illuminated egg microbiome origins, which suggested a limited potential of eggshells for transgenerational transmission, encouraging further investigation of eggshell microbiome functions in vertebrates.

KW - TRANS-SHELL INFECTION

KW - GUT MICROBIOTA

KW - EGG VIABILITY

KW - BACTERIAL

KW - INCUBATION

KW - LIFE

KW - TRANSMISSION

KW - COMMUNITIES

KW - DIVERSITY

KW - EVOLUTION

U2 - 10.1038/s41396-018-0067-3

DO - 10.1038/s41396-018-0067-3

M3 - Article

VL - 12

SP - 1375

EP - 1388

JO - ISME Journal

JF - ISME Journal

SN - 1751-7370

IS - 5

ER -

ID: 54548116