Publication

Micro Versus Macro: The Effect of Environmental Confinement on Cellular Nanoparticle Uptake

Damle, V. G., Sharmin, R., Morita, A., Nie, L. & Schirhagl, R., 24-Jul-2020, In : Frontiers in Bioengineering and Biotechnology. 8, 13 p., 869.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Damle, V. G., Sharmin, R., Morita, A., Nie, L., & Schirhagl, R. (2020). Micro Versus Macro: The Effect of Environmental Confinement on Cellular Nanoparticle Uptake. Frontiers in Bioengineering and Biotechnology, 8, [869]. https://doi.org/10.3389/fbioe.2020.00869

Author

Damle, Viraj G. ; Sharmin, Rokshana ; Morita, Aryan ; Nie, Linyan ; Schirhagl, Romana. / Micro Versus Macro : The Effect of Environmental Confinement on Cellular Nanoparticle Uptake. In: Frontiers in Bioengineering and Biotechnology. 2020 ; Vol. 8.

Harvard

Damle, VG, Sharmin, R, Morita, A, Nie, L & Schirhagl, R 2020, 'Micro Versus Macro: The Effect of Environmental Confinement on Cellular Nanoparticle Uptake', Frontiers in Bioengineering and Biotechnology, vol. 8, 869. https://doi.org/10.3389/fbioe.2020.00869

Standard

Micro Versus Macro : The Effect of Environmental Confinement on Cellular Nanoparticle Uptake. / Damle, Viraj G.; Sharmin, Rokshana; Morita, Aryan; Nie, Linyan; Schirhagl, Romana.

In: Frontiers in Bioengineering and Biotechnology, Vol. 8, 869, 24.07.2020.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Damle VG, Sharmin R, Morita A, Nie L, Schirhagl R. Micro Versus Macro: The Effect of Environmental Confinement on Cellular Nanoparticle Uptake. Frontiers in Bioengineering and Biotechnology. 2020 Jul 24;8. 869. https://doi.org/10.3389/fbioe.2020.00869


BibTeX

@article{8bc41b5f1abc4da7aac161f2a2b55035,
title = "Micro Versus Macro: The Effect of Environmental Confinement on Cellular Nanoparticle Uptake",
abstract = "While the microenvironment is known to alter the cellular behavior in terms of metabolism, growth and the degree of endoplasmic reticulum stress, its influence on the nanoparticle uptake is not yet investigated. Specifically, it is not clear if the cells cultured in a microenvironment ingest different amounts of nanoparticles than cells cultured in a macroenvironment (for example a petri dish). To answer this question, here we used J774 murine macrophages and fluorescent nanodiamonds (FND) as a model system to systematically compare the uptake efficiency of cells cultured in a petri dish and in a microfluidic channel. Specifically, equal numbers of cells were cultured in two devices followed by the FND incubation. Then cells were fixed, stained and imaged to quantify the FND uptake. We show that the FND uptake in the cells cultured in petri dishes is significantly higher than the uptake in a microfluidic chip where the alteration in CO(2)environment, the cell culture medium pH and the surface area to volume ratio seem to be the underlying causes leading to this observed difference.",
keywords = "microfluidic cell cultures, nanoparticles, fluorescent nanodiamonds, macrophages, cellular uptake, NITROGEN-VACANCY CENTERS, DRUG-DELIVERY, FLUORESCENT NANODIAMONDS, CELLS, SHAPE, MICROFLUIDICS, CARRIERS, CHIP, SIZE",
author = "Damle, {Viraj G.} and Rokshana Sharmin and Aryan Morita and Linyan Nie and Romana Schirhagl",
year = "2020",
month = jul,
day = "24",
doi = "10.3389/fbioe.2020.00869",
language = "English",
volume = "8",
journal = "Frontiers in Bioengineering and Biotechnology",
issn = "2296-4185",
publisher = "Frontiers Media SA",

}

RIS

TY - JOUR

T1 - Micro Versus Macro

T2 - The Effect of Environmental Confinement on Cellular Nanoparticle Uptake

AU - Damle, Viraj G.

AU - Sharmin, Rokshana

AU - Morita, Aryan

AU - Nie, Linyan

AU - Schirhagl, Romana

PY - 2020/7/24

Y1 - 2020/7/24

N2 - While the microenvironment is known to alter the cellular behavior in terms of metabolism, growth and the degree of endoplasmic reticulum stress, its influence on the nanoparticle uptake is not yet investigated. Specifically, it is not clear if the cells cultured in a microenvironment ingest different amounts of nanoparticles than cells cultured in a macroenvironment (for example a petri dish). To answer this question, here we used J774 murine macrophages and fluorescent nanodiamonds (FND) as a model system to systematically compare the uptake efficiency of cells cultured in a petri dish and in a microfluidic channel. Specifically, equal numbers of cells were cultured in two devices followed by the FND incubation. Then cells were fixed, stained and imaged to quantify the FND uptake. We show that the FND uptake in the cells cultured in petri dishes is significantly higher than the uptake in a microfluidic chip where the alteration in CO(2)environment, the cell culture medium pH and the surface area to volume ratio seem to be the underlying causes leading to this observed difference.

AB - While the microenvironment is known to alter the cellular behavior in terms of metabolism, growth and the degree of endoplasmic reticulum stress, its influence on the nanoparticle uptake is not yet investigated. Specifically, it is not clear if the cells cultured in a microenvironment ingest different amounts of nanoparticles than cells cultured in a macroenvironment (for example a petri dish). To answer this question, here we used J774 murine macrophages and fluorescent nanodiamonds (FND) as a model system to systematically compare the uptake efficiency of cells cultured in a petri dish and in a microfluidic channel. Specifically, equal numbers of cells were cultured in two devices followed by the FND incubation. Then cells were fixed, stained and imaged to quantify the FND uptake. We show that the FND uptake in the cells cultured in petri dishes is significantly higher than the uptake in a microfluidic chip where the alteration in CO(2)environment, the cell culture medium pH and the surface area to volume ratio seem to be the underlying causes leading to this observed difference.

KW - microfluidic cell cultures

KW - nanoparticles

KW - fluorescent nanodiamonds

KW - macrophages

KW - cellular uptake

KW - NITROGEN-VACANCY CENTERS

KW - DRUG-DELIVERY

KW - FLUORESCENT NANODIAMONDS

KW - CELLS

KW - SHAPE

KW - MICROFLUIDICS

KW - CARRIERS

KW - CHIP

KW - SIZE

U2 - 10.3389/fbioe.2020.00869

DO - 10.3389/fbioe.2020.00869

M3 - Article

VL - 8

JO - Frontiers in Bioengineering and Biotechnology

JF - Frontiers in Bioengineering and Biotechnology

SN - 2296-4185

M1 - 869

ER -

ID: 133475296