MHC class II genes in the European badger (Meles meles): Characterization, patterns of variation, and transcription analysisSin, Y. W., Dugdale, H. L., Newman, C., Macdonald, D. W. & Burke, T., Apr-2012, In : Immunogenetics. 64, 4, p. 313-327 15 p.
Research output: Contribution to journal › Article › Academic › peer-review
The major histocompatibility complex (MHC) comprises many genes, some of which are polymorphic with numerous alleles. Sequence variation among alleles is most pronounced in exon 2 of the class II genes, which encodes the alpha 1 and beta 1 domains that form the antigen-binding site (ABS) for the presentation of peptides. The MHC thus plays an important role in pathogen defense. European badgers (Meles meles) are a good species in which to study the MHC, as they harbor a variety of pathogens. We present the first characterization of MHC class II genes, isolated from genomic DNA (gDNA) and complementary DNA (cDNA), in the European badger. Examination of seven individuals revealed four DRB, two DQB, two DQA, and two DRA putatively functional gDNA sequences. All of these sequences, except DRA, exhibited high variability in exon 2; DRB had the highest variability. The ABS codons demonstrated high variability, due potentially to balancing selection, while non-ABS codons had lower variability. Positively selected sites were detected in DRB and DQA. Phylogenetic analysis demonstrated trans-species polymorphism of class II genes. Comparison with cDNA from whole blood revealed that only DRB had a transcription pattern reflecting the alleles that were present in the gDNA, while the other three genes had disparities between gDNA and cDNA. Only one sequence was transcribed, even though two gDNA sequences were present, from each of both DQB and DRA. Our characterization of badger MHC sequences forms a basis for further studies of MHC variability, mate choice, and pathogen resistance in this, and other, species.
|Number of pages||15|
|Publication status||Published - Apr-2012|
- Balancing selection, DRB gene, Immunogenetics, Mustelid, Pathogen resistance, Trans-species polymorphism, MAJOR HISTOCOMPATIBILITY COMPLEX, LION ZALOPHUS-CALIFORNIANUS, EURASIAN BADGER, DIFFERENTIAL EXPRESSION, NATURAL-SELECTION, MOLECULAR POLYMORPHISM, EVOLUTIONARY HISTORY, POPULATION-DYNAMICS, RHESUS MACAQUES, SOCIAL-GROUPS