Methods for providing sialylated oligosaccharides

Sallomons, E., Wilbrink, M. H., Sanders, P. & Kamerling, J. P., 13-Jun-2013, Patent No. WO2013085384, 6-Dec-2012, Priority date 7-Dec-2011

Research output: Patent

  • Erik Sallomons (Inventor)
  • Maarten Hotse Wilbrink (Inventor)
  • Peter Sanders (Inventor)
  • Johannis Paulis Kamerling (Inventor)
The invention relates to a method for providing analogs of human milk oligosaccharides (HMO), in particular oligosaccharides containing terminal sialic acid. The method comprises the steps of : a) providing a source of non-digestible galactooligosaccharides (GOS) containing at least two terminally bonded b-linked galactose residues; b) providing a sialic acid donor having (a2-3)-sialylated O-glycans; c) contacting said GOS with said sialic acid donor in the presence of an enzyme having trans-sialidase activity in an enzyme reaction mixture; and d) isolating from said enzyme reaction mixture a fraction comprising at least 20 percent by weight of disialylated galactooligosaccharides (di-Sia-GOS) based on the dry matter.
Original languageEnglish
Patent numberWO2013085384
Priority date07/12/2011
Filing date06/12/2012
Publication statusPublished - 13-Jun-2013

ID: 87509596