Publication

Metformin preconditioning improves hepatobiliary function and reduces injury in a rat model of normothermic machine perfusion and orthotopic transplantation

Westerkamp, A. C., Fujiyoshi, M., Ottens, P. J., Nijsten, M. W. N., Touw, D. J., de Meijer, V. E., Lisman, T., Leuvenink, H. G. D., Moshage, H., Berendsen, T. A. & Porte, R. J., 2-Mar-2020, In : Transplantation. 35 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Westerkamp, A. C., Fujiyoshi, M., Ottens, P. J., Nijsten, M. W. N., Touw, D. J., de Meijer, V. E., ... Porte, R. J. (2020). Metformin preconditioning improves hepatobiliary function and reduces injury in a rat model of normothermic machine perfusion and orthotopic transplantation. Transplantation. https://doi.org/10.1097/TP.0000000000003216

Author

Westerkamp, Andrie C ; Fujiyoshi, Masato ; Ottens, Petra J ; Nijsten, Maarten W N ; Touw, Daan J ; de Meijer, Vincent E ; Lisman, Ton ; Leuvenink, Henri G D ; Moshage, Han ; Berendsen, Tim A ; Porte, Robert J. / Metformin preconditioning improves hepatobiliary function and reduces injury in a rat model of normothermic machine perfusion and orthotopic transplantation. In: Transplantation. 2020.

Harvard

Westerkamp, AC, Fujiyoshi, M, Ottens, PJ, Nijsten, MWN, Touw, DJ, de Meijer, VE, Lisman, T, Leuvenink, HGD, Moshage, H, Berendsen, TA & Porte, RJ 2020, 'Metformin preconditioning improves hepatobiliary function and reduces injury in a rat model of normothermic machine perfusion and orthotopic transplantation', Transplantation. https://doi.org/10.1097/TP.0000000000003216

Standard

Metformin preconditioning improves hepatobiliary function and reduces injury in a rat model of normothermic machine perfusion and orthotopic transplantation. / Westerkamp, Andrie C; Fujiyoshi, Masato; Ottens, Petra J; Nijsten, Maarten W N; Touw, Daan J; de Meijer, Vincent E; Lisman, Ton; Leuvenink, Henri G D; Moshage, Han; Berendsen, Tim A; Porte, Robert J.

In: Transplantation, 02.03.2020.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Westerkamp AC, Fujiyoshi M, Ottens PJ, Nijsten MWN, Touw DJ, de Meijer VE et al. Metformin preconditioning improves hepatobiliary function and reduces injury in a rat model of normothermic machine perfusion and orthotopic transplantation. Transplantation. 2020 Mar 2. https://doi.org/10.1097/TP.0000000000003216


BibTeX

@article{fc607fb613d24e0f8db90bf755236a85,
title = "Metformin preconditioning improves hepatobiliary function and reduces injury in a rat model of normothermic machine perfusion and orthotopic transplantation",
abstract = "BACKGROUND: Preconditioning of donor livers prior to organ retrieval may improve organ quality after transplantation. We investigated whether preconditioning with metformin reduces preservation injury and improves hepatobiliary function in rat donor livers during ex situ normothermic machine perfusion (NMP) and after orthotopic liver transplantation.METHODS: Lewis rats were administered metformin via oral gavage, after which a donor hepatectomy was performed followed by a standardized cold storage period of 4 hours. Graft assessment was carried out using NMP via double perfusion of the hepatic artery and portal vein. In an additional experiment, rat donor livers preconditioned with metformin were stored on ice for 4 hours and transplanted to confirm postoperative liver function and survival. Data was analyzed and compared with sham-fed controls.RESULTS: Graft assessment using NMP confirmed that preconditioning significantly improved ATP production, markers for hepatobiliary function (total bile production, biliary bilirubin and bicarbonate), and significantly lowered levels of lactate, glucose and apoptosis. After orthotopic liver transplantation, metformin preconditioning significantly reduced transaminase levels.CONCLUSION: Preconditioning with metformin lowers hepatobiliary injury and improves hepatobiliary function in an in situ and ex situ model of rat donor liver transplantation.",
author = "Westerkamp, {Andrie C} and Masato Fujiyoshi and Ottens, {Petra J} and Nijsten, {Maarten W N} and Touw, {Daan J} and {de Meijer}, {Vincent E} and Ton Lisman and Leuvenink, {Henri G D} and Han Moshage and Berendsen, {Tim A} and Porte, {Robert J}",
year = "2020",
month = "3",
day = "2",
doi = "10.1097/TP.0000000000003216",
language = "English",
journal = "Transplantation",
issn = "0041-1337",
publisher = "LIPPINCOTT WILLIAMS & WILKINS",

}

RIS

TY - JOUR

T1 - Metformin preconditioning improves hepatobiliary function and reduces injury in a rat model of normothermic machine perfusion and orthotopic transplantation

AU - Westerkamp, Andrie C

AU - Fujiyoshi, Masato

AU - Ottens, Petra J

AU - Nijsten, Maarten W N

AU - Touw, Daan J

AU - de Meijer, Vincent E

AU - Lisman, Ton

AU - Leuvenink, Henri G D

AU - Moshage, Han

AU - Berendsen, Tim A

AU - Porte, Robert J

PY - 2020/3/2

Y1 - 2020/3/2

N2 - BACKGROUND: Preconditioning of donor livers prior to organ retrieval may improve organ quality after transplantation. We investigated whether preconditioning with metformin reduces preservation injury and improves hepatobiliary function in rat donor livers during ex situ normothermic machine perfusion (NMP) and after orthotopic liver transplantation.METHODS: Lewis rats were administered metformin via oral gavage, after which a donor hepatectomy was performed followed by a standardized cold storage period of 4 hours. Graft assessment was carried out using NMP via double perfusion of the hepatic artery and portal vein. In an additional experiment, rat donor livers preconditioned with metformin were stored on ice for 4 hours and transplanted to confirm postoperative liver function and survival. Data was analyzed and compared with sham-fed controls.RESULTS: Graft assessment using NMP confirmed that preconditioning significantly improved ATP production, markers for hepatobiliary function (total bile production, biliary bilirubin and bicarbonate), and significantly lowered levels of lactate, glucose and apoptosis. After orthotopic liver transplantation, metformin preconditioning significantly reduced transaminase levels.CONCLUSION: Preconditioning with metformin lowers hepatobiliary injury and improves hepatobiliary function in an in situ and ex situ model of rat donor liver transplantation.

AB - BACKGROUND: Preconditioning of donor livers prior to organ retrieval may improve organ quality after transplantation. We investigated whether preconditioning with metformin reduces preservation injury and improves hepatobiliary function in rat donor livers during ex situ normothermic machine perfusion (NMP) and after orthotopic liver transplantation.METHODS: Lewis rats were administered metformin via oral gavage, after which a donor hepatectomy was performed followed by a standardized cold storage period of 4 hours. Graft assessment was carried out using NMP via double perfusion of the hepatic artery and portal vein. In an additional experiment, rat donor livers preconditioned with metformin were stored on ice for 4 hours and transplanted to confirm postoperative liver function and survival. Data was analyzed and compared with sham-fed controls.RESULTS: Graft assessment using NMP confirmed that preconditioning significantly improved ATP production, markers for hepatobiliary function (total bile production, biliary bilirubin and bicarbonate), and significantly lowered levels of lactate, glucose and apoptosis. After orthotopic liver transplantation, metformin preconditioning significantly reduced transaminase levels.CONCLUSION: Preconditioning with metformin lowers hepatobiliary injury and improves hepatobiliary function in an in situ and ex situ model of rat donor liver transplantation.

U2 - 10.1097/TP.0000000000003216

DO - 10.1097/TP.0000000000003216

M3 - Article

JO - Transplantation

JF - Transplantation

SN - 0041-1337

ER -

ID: 120082243