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Metabolic Engineering toward Sustainable Production of Nylon-6

Turk, S. C. H. J., Kloosterman, W. P., Ninaber, D. K., Kolen, K. P. A. M., Knutova, J., Suir, E., Schurmann, M., Raemakers-Franken, P. C., Muller, M., de Wildeman, S. M. A., Raamsdonk, L. M., van der Pol, R., Wu, L., Temudo, M. F., van der Hoeven, R. A. M., Akeroyd, M., van der Stoel, R. E., Noorman, H. J., Bovenberg, R. A. L. & Trefzer, A. C., Jan-2016, In : ACS Synthetic Biology. 5, 1, p. 65-73 9 p.

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  • Metabolic Engineering toward Sustainable Production of Nylon‑6

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DOI

  • Stefan C. H. J. Turk
  • Wigard P. Kloosterman
  • Dennis K. Ninaber
  • Karin P. A. M. Kolen
  • Julia Knutova
  • Erwin Suir
  • Martin Schurmann
  • Petronella C. Raemakers-Franken
  • Monika Muller
  • Stefaan M. A. de Wildeman
  • Leonie M. Raamsdonk
  • Ruud van der Pol
  • Liang Wu
  • Margarida F. Temudo
  • Rob A. M. van der Hoeven
  • Michiel Akeroyd
  • Roland E. van der Stoel
  • Henk J. Noorman
  • Roel A. L. Bovenberg
  • Axel C. Trefzer

Nylon-6 is a bulk polymer used for many applications. It consists of the non-natural building block 6-aminocaproic acid, the linear form of caprolactam. Via a retro-synthetic approach, two synthetic pathways were identified for the fermentative production of 6-aminocaproic acid. Both pathways require yet unreported novel biocatalytic steps. We demonstrated proof of these bioconversions by in vitro enzyme assays with a set of selected candidate proteins expressed in Escherichia coli. One of the biosynthetic pathways starts with 2-oxoglutarate and contains bioconversions of the ketoacid elongation pathway known from methanogenic archaea. This pathway was selected for implementation in E. coli and yielded 6-aminocaproic acid at levels up to 160 mg/L in lab-scale batch fermentations. The total amount of 6-aminocaproic acid and related intermediates generated by this pathway exceeded 2 g/L in lab-scale fed-batch fermentations, indicating its potential for further optimization toward large-scale sustainable production of nylon-6.

Original languageEnglish
Pages (from-to)65-73
Number of pages9
JournalACS Synthetic Biology
Volume5
Issue number1
Publication statusPublished - Jan-2016

    Keywords

  • nylon-6, caprolactam, metabolic engineering, 6-aminocaproic acid, alpha-ketopimelate, adipate, ADIPIC ACID, COENZYME B, CORYNEBACTERIUM-GLUTAMICUM, PROTEIN EXPRESSION, ESCHERICHIA-COLI, BULK CHEMICALS, AMINO-ACIDS, CLONING, BIOSYNTHESIS, IDENTIFICATION

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