Publication

Metabolic cross-talk between human bronchial epithelial cells and internalized Staphylococcus aureus as a driver for infection

Palma Medina, L. M., Becker, A-K., Michalik, S., Yedavally, H., Raineri, E. J. M., Hildebrandt, P., Gesell Salazar, M., Surmann, K., Pförtner, H., Mekonnen, S. A., Salvati, A., Kaderali, L., van Dijl, J. M. & Völker, U., May-2019, In : Molecular & Cellular Proteomics. 18, 5, p. 892-908 17 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Palma Medina, L. M., Becker, A-K., Michalik, S., Yedavally, H., Raineri, E. J. M., Hildebrandt, P., ... Völker, U. (2019). Metabolic cross-talk between human bronchial epithelial cells and internalized Staphylococcus aureus as a driver for infection. Molecular & Cellular Proteomics, 18(5), 892-908. https://doi.org/10.1074/mcp.RA118.001138

Author

Palma Medina, Laura M ; Becker, Ann-Kristin ; Michalik, Stephan ; Yedavally, Harita ; Raineri, Elisa J M ; Hildebrandt, Petra ; Gesell Salazar, Manuela ; Surmann, Kristin ; Pförtner, Henrike ; Mekonnen, Solomon A ; Salvati, Anna ; Kaderali, Lars ; van Dijl, Jan Maarten ; Völker, Uwe. / Metabolic cross-talk between human bronchial epithelial cells and internalized Staphylococcus aureus as a driver for infection. In: Molecular & Cellular Proteomics. 2019 ; Vol. 18, No. 5. pp. 892-908.

Harvard

Palma Medina, LM, Becker, A-K, Michalik, S, Yedavally, H, Raineri, EJM, Hildebrandt, P, Gesell Salazar, M, Surmann, K, Pförtner, H, Mekonnen, SA, Salvati, A, Kaderali, L, van Dijl, JM & Völker, U 2019, 'Metabolic cross-talk between human bronchial epithelial cells and internalized Staphylococcus aureus as a driver for infection', Molecular & Cellular Proteomics, vol. 18, no. 5, pp. 892-908. https://doi.org/10.1074/mcp.RA118.001138

Standard

Metabolic cross-talk between human bronchial epithelial cells and internalized Staphylococcus aureus as a driver for infection. / Palma Medina, Laura M; Becker, Ann-Kristin; Michalik, Stephan; Yedavally, Harita; Raineri, Elisa J M; Hildebrandt, Petra; Gesell Salazar, Manuela; Surmann, Kristin; Pförtner, Henrike; Mekonnen, Solomon A; Salvati, Anna; Kaderali, Lars; van Dijl, Jan Maarten; Völker, Uwe.

In: Molecular & Cellular Proteomics, Vol. 18, No. 5, 05.2019, p. 892-908.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Palma Medina LM, Becker A-K, Michalik S, Yedavally H, Raineri EJM, Hildebrandt P et al. Metabolic cross-talk between human bronchial epithelial cells and internalized Staphylococcus aureus as a driver for infection. Molecular & Cellular Proteomics. 2019 May;18(5):892-908. https://doi.org/10.1074/mcp.RA118.001138


BibTeX

@article{5cd91bd317c9470281e416a850655259,
title = "Metabolic cross-talk between human bronchial epithelial cells and internalized Staphylococcus aureus as a driver for infection",
abstract = "Staphylococcus aureus is infamous for causing recurrent infections of the human respiratory tract. This is a consequence of its ability to adapt to different niches, including the intracellular milieu of lung epithelial cells. To understand the dynamic interplay between epithelial cells and the intracellular pathogen, we dissected their interactions over four days by mass spectrometry. Additionally, we investigated the dynamics of infection through live cell imaging, immunofluorescence and electron microscopy. The results highlight a major role of often overlooked temporal changes in the bacterial and host metabolism, triggered by fierce competition over limited resources. Remarkably, replicating bacteria reside predominantly within membrane-enclosed compartments and induce apoptosis of the host within ~24 hours post infection. Surviving infected host cells carry a subpopulation of non-replicating bacteria in the cytoplasm that persists. Altogether, we conclude that, besides the production of virulence factors by bacteria, it is the way in which intracellular resources are used, and how host and intracellular bacteria subsequently adapt to each other that determines the ultimate outcome of the infectious process.",
author = "{Palma Medina}, {Laura M} and Ann-Kristin Becker and Stephan Michalik and Harita Yedavally and Raineri, {Elisa J M} and Petra Hildebrandt and {Gesell Salazar}, Manuela and Kristin Surmann and Henrike Pf{\"o}rtner and Mekonnen, {Solomon A} and Anna Salvati and Lars Kaderali and {van Dijl}, {Jan Maarten} and Uwe V{\"o}lker",
note = "Published under license by The American Society for Biochemistry and Molecular Biology, Inc.",
year = "2019",
month = "5",
doi = "10.1074/mcp.RA118.001138",
language = "English",
volume = "18",
pages = "892--908",
journal = "Molecular & Cellular Proteomics",
issn = "1535-9476",
publisher = "AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC",
number = "5",

}

RIS

TY - JOUR

T1 - Metabolic cross-talk between human bronchial epithelial cells and internalized Staphylococcus aureus as a driver for infection

AU - Palma Medina, Laura M

AU - Becker, Ann-Kristin

AU - Michalik, Stephan

AU - Yedavally, Harita

AU - Raineri, Elisa J M

AU - Hildebrandt, Petra

AU - Gesell Salazar, Manuela

AU - Surmann, Kristin

AU - Pförtner, Henrike

AU - Mekonnen, Solomon A

AU - Salvati, Anna

AU - Kaderali, Lars

AU - van Dijl, Jan Maarten

AU - Völker, Uwe

N1 - Published under license by The American Society for Biochemistry and Molecular Biology, Inc.

PY - 2019/5

Y1 - 2019/5

N2 - Staphylococcus aureus is infamous for causing recurrent infections of the human respiratory tract. This is a consequence of its ability to adapt to different niches, including the intracellular milieu of lung epithelial cells. To understand the dynamic interplay between epithelial cells and the intracellular pathogen, we dissected their interactions over four days by mass spectrometry. Additionally, we investigated the dynamics of infection through live cell imaging, immunofluorescence and electron microscopy. The results highlight a major role of often overlooked temporal changes in the bacterial and host metabolism, triggered by fierce competition over limited resources. Remarkably, replicating bacteria reside predominantly within membrane-enclosed compartments and induce apoptosis of the host within ~24 hours post infection. Surviving infected host cells carry a subpopulation of non-replicating bacteria in the cytoplasm that persists. Altogether, we conclude that, besides the production of virulence factors by bacteria, it is the way in which intracellular resources are used, and how host and intracellular bacteria subsequently adapt to each other that determines the ultimate outcome of the infectious process.

AB - Staphylococcus aureus is infamous for causing recurrent infections of the human respiratory tract. This is a consequence of its ability to adapt to different niches, including the intracellular milieu of lung epithelial cells. To understand the dynamic interplay between epithelial cells and the intracellular pathogen, we dissected their interactions over four days by mass spectrometry. Additionally, we investigated the dynamics of infection through live cell imaging, immunofluorescence and electron microscopy. The results highlight a major role of often overlooked temporal changes in the bacterial and host metabolism, triggered by fierce competition over limited resources. Remarkably, replicating bacteria reside predominantly within membrane-enclosed compartments and induce apoptosis of the host within ~24 hours post infection. Surviving infected host cells carry a subpopulation of non-replicating bacteria in the cytoplasm that persists. Altogether, we conclude that, besides the production of virulence factors by bacteria, it is the way in which intracellular resources are used, and how host and intracellular bacteria subsequently adapt to each other that determines the ultimate outcome of the infectious process.

U2 - 10.1074/mcp.RA118.001138

DO - 10.1074/mcp.RA118.001138

M3 - Article

VL - 18

SP - 892

EP - 908

JO - Molecular & Cellular Proteomics

JF - Molecular & Cellular Proteomics

SN - 1535-9476

IS - 5

ER -

ID: 77423590