Publication

Mechanistic aspects of uptake and sinusoidal efflux of dibromosulfophthalein in the isolated perfused rat liver

Nijssen, H. M. J., Pijning, T., Meijer, D. K. F. & Groothuis, G. M. M., 24-Oct-1991, In : Biochemical Pharmacology. 42, 10, p. 1997-2002 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Nijssen, H. M. J., Pijning, T., Meijer, D. K. F., & Groothuis, G. M. M. (1991). Mechanistic aspects of uptake and sinusoidal efflux of dibromosulfophthalein in the isolated perfused rat liver. Biochemical Pharmacology, 42(10), 1997-2002. https://doi.org/10.1016/0006-2952(91)90600-A

Author

Nijssen, H.M.J ; Pijning, T ; Meijer, D.K.F ; Groothuis, G.M.M. / Mechanistic aspects of uptake and sinusoidal efflux of dibromosulfophthalein in the isolated perfused rat liver. In: Biochemical Pharmacology. 1991 ; Vol. 42, No. 10. pp. 1997-2002.

Harvard

Nijssen, HMJ, Pijning, T, Meijer, DKF & Groothuis, GMM 1991, 'Mechanistic aspects of uptake and sinusoidal efflux of dibromosulfophthalein in the isolated perfused rat liver', Biochemical Pharmacology, vol. 42, no. 10, pp. 1997-2002. https://doi.org/10.1016/0006-2952(91)90600-A

Standard

Mechanistic aspects of uptake and sinusoidal efflux of dibromosulfophthalein in the isolated perfused rat liver. / Nijssen, H.M.J; Pijning, T; Meijer, D.K.F; Groothuis, G.M.M.

In: Biochemical Pharmacology, Vol. 42, No. 10, 24.10.1991, p. 1997-2002.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Nijssen HMJ, Pijning T, Meijer DKF, Groothuis GMM. Mechanistic aspects of uptake and sinusoidal efflux of dibromosulfophthalein in the isolated perfused rat liver. Biochemical Pharmacology. 1991 Oct 24;42(10):1997-2002. https://doi.org/10.1016/0006-2952(91)90600-A


BibTeX

@article{a114a8236bc047b0b21066f100572e0b,
title = "Mechanistic aspects of uptake and sinusoidal efflux of dibromosulfophthalein in the isolated perfused rat liver",
abstract = "Using the isolated perfused rat liver technique we examined whether the accumulation and sinusoidal efflux processes of the organic anion dibromosulfophthalein (DBSP) are dependent on the intracellular ATP content, chloride concentration in the perfusion medium as well as temperature of the medium and whether they are mediated by the same or by separate carrier mechanisms. The net sinusoidal efflux rate, being the resultant of sinusoidal efflux and re-uptake, was decreased more than 50% after lowering the medium temperature from 37 to 26-degrees indicating that the efflux process is carrier-mediated. The uptake rate was decreased only 18% after lowering the medium temperature to 26-degrees. Lowering of the hepatic ATP content for more than 80% clearly decreased the DBSP uptake rate but not the sinusoidal efflux rate. These observations indicate that these opposing transport steps probably are mediated by two separate carriers. Additional evidence for this hypothesis originated from the observation that sinusoidal efflux of DBSP was decreased about 30% whereas hepatic uptake of the substrate remained unaltered after replacing chloride in the perfusion medium with gluconate. In summary, we conclude that uptake and sinusoidal efflux of DBSP are mediated by two separate carrier systems that are influenced differently by ATP depletion, temperature lowering and presence of Cl- gradients.",
keywords = "HEPATOCELLULAR UPTAKE, ORGANIC-ANIONS, MEMBRANE-VESICLES, HEPATOCYTES, TRANSPORT, SULFOBROMOPHTHALEIN, CELLS",
author = "H.M.J Nijssen and T Pijning and D.K.F Meijer and G.M.M Groothuis",
year = "1991",
month = oct,
day = "24",
doi = "10.1016/0006-2952(91)90600-A",
language = "English",
volume = "42",
pages = "1997--2002",
journal = "Biochemical Pharmacology",
issn = "0006-2952",
publisher = "PERGAMON-ELSEVIER SCIENCE LTD",
number = "10",

}

RIS

TY - JOUR

T1 - Mechanistic aspects of uptake and sinusoidal efflux of dibromosulfophthalein in the isolated perfused rat liver

AU - Nijssen, H.M.J

AU - Pijning, T

AU - Meijer, D.K.F

AU - Groothuis, G.M.M

PY - 1991/10/24

Y1 - 1991/10/24

N2 - Using the isolated perfused rat liver technique we examined whether the accumulation and sinusoidal efflux processes of the organic anion dibromosulfophthalein (DBSP) are dependent on the intracellular ATP content, chloride concentration in the perfusion medium as well as temperature of the medium and whether they are mediated by the same or by separate carrier mechanisms. The net sinusoidal efflux rate, being the resultant of sinusoidal efflux and re-uptake, was decreased more than 50% after lowering the medium temperature from 37 to 26-degrees indicating that the efflux process is carrier-mediated. The uptake rate was decreased only 18% after lowering the medium temperature to 26-degrees. Lowering of the hepatic ATP content for more than 80% clearly decreased the DBSP uptake rate but not the sinusoidal efflux rate. These observations indicate that these opposing transport steps probably are mediated by two separate carriers. Additional evidence for this hypothesis originated from the observation that sinusoidal efflux of DBSP was decreased about 30% whereas hepatic uptake of the substrate remained unaltered after replacing chloride in the perfusion medium with gluconate. In summary, we conclude that uptake and sinusoidal efflux of DBSP are mediated by two separate carrier systems that are influenced differently by ATP depletion, temperature lowering and presence of Cl- gradients.

AB - Using the isolated perfused rat liver technique we examined whether the accumulation and sinusoidal efflux processes of the organic anion dibromosulfophthalein (DBSP) are dependent on the intracellular ATP content, chloride concentration in the perfusion medium as well as temperature of the medium and whether they are mediated by the same or by separate carrier mechanisms. The net sinusoidal efflux rate, being the resultant of sinusoidal efflux and re-uptake, was decreased more than 50% after lowering the medium temperature from 37 to 26-degrees indicating that the efflux process is carrier-mediated. The uptake rate was decreased only 18% after lowering the medium temperature to 26-degrees. Lowering of the hepatic ATP content for more than 80% clearly decreased the DBSP uptake rate but not the sinusoidal efflux rate. These observations indicate that these opposing transport steps probably are mediated by two separate carriers. Additional evidence for this hypothesis originated from the observation that sinusoidal efflux of DBSP was decreased about 30% whereas hepatic uptake of the substrate remained unaltered after replacing chloride in the perfusion medium with gluconate. In summary, we conclude that uptake and sinusoidal efflux of DBSP are mediated by two separate carrier systems that are influenced differently by ATP depletion, temperature lowering and presence of Cl- gradients.

KW - HEPATOCELLULAR UPTAKE

KW - ORGANIC-ANIONS

KW - MEMBRANE-VESICLES

KW - HEPATOCYTES

KW - TRANSPORT

KW - SULFOBROMOPHTHALEIN

KW - CELLS

U2 - 10.1016/0006-2952(91)90600-A

DO - 10.1016/0006-2952(91)90600-A

M3 - Article

VL - 42

SP - 1997

EP - 2002

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

SN - 0006-2952

IS - 10

ER -

ID: 6299670