Publication

Mechanisms of influenza viral membrane fusion

Blijleven, J. S., Boonstra, S., Onck, P. R., van der Giessen, E. & van Oijen, A. M., Dec-2016, In : Seminars in Cell & Developmental Biology. 60, p. 78-88 11 p.

Research output: Contribution to journalReview articleAcademicpeer-review

Influenza viral particles are enveloped by a lipid bilayer. A major step in infection is fusion of the viral and host cellular membranes, a process with large kinetic barriers. Influenza membrane fusion is catalyzed by hemagglutinin (HA), a class I viral fusion protein activated by low pH. The exact nature of the HA conformational changes that deliver the energy required for fusion remains poorly understood. This review summarizes our current knowledge of HA structure and dynamics, describes recent single-particle experiments and modeling studies, and discusses their role in understanding how multiple HAs mediate fusion. These approaches provide a mechanistic picture in which HAs independently and stochastically insert into the target membrane, forming a cluster of HAs that is collectively able to overcome the barrier to membrane fusion. The new experimental and modeling approaches described in this review hold promise for a more complete understanding of other viral fusion systems and the protein systems responsible for cellular fusion.

Original languageEnglish
Pages (from-to)78-88
Number of pages11
JournalSeminars in Cell & Developmental Biology
Volume60
Publication statusPublished - Dec-2016

    Keywords

  • TRANSMEMBRANE DOMAIN, RECEPTOR-BINDING, HEMAGGLUTININ SURFACE-DENSITY, INDUCED CONFORMATIONAL-CHANGE, PLANAR BILAYER-MEMBRANES, PH-DEPENDENT FUSION, VIRUS TYPE-1 GP41, CELL-CELL FUSION, SINGLE-PARTICLE, SIALIC-ACID, Structure , Modeling, Influenza, Hemagglutinin, Membrane fusion, Single-particle

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