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Measurement of brown adipose tissue mass using a novel dual-echo magnetic resonance imaging approach: A validation study

Holstila, M., Virtanen, K. A., Gronroos, T. J., Laine, J., Lepomaki, V., Saunavaara, J., Lisinen, I., Komu, M., Hannukainen, L. C., Nuutila, P., Parkkola, R. & Borra, R. J. H., Aug-2013, In : METABOLISM-CLINICAL AND EXPERIMENTAL. 62, 8, p. 1189-1198 10 p.

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  • Measurement of brown adipose tissue mass using a novel dual-echo magnetic resonance imaging approach: A validation study

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DOI

  • Milja Holstila
  • Kirsi A. Virtanen
  • Tove J. Gronroos
  • Jukka Laine
  • Virva Lepomaki
  • Jani Saunavaara
  • Irina Lisinen
  • Markku Komu
  • Lama C. Hannukainen
  • Pirjo Nuutila
  • Riitta Parkkola
  • Ronald J. H. Borra

Objective. The aim of this study was to evaluate and validate magnetic resonance imaging (MRI) for the visualization and quantification of brown adipose tissue (BAT) in vivo in a rat model. We hypothesized that, based on differences in tissue water and lipid content, MRI could reliably differentiate between BAT and white adipose tissue (WAT) and could therefore be a possible alternative for 18 F-Fluorodeoxyglucose Positron Emission Tomography ((18)FDG-PET), the current gold standard for non-invasive BAT quantification.

Materials/Methods. Eleven rats were studied using both (18)FDG-PET/CT and MRI (1.5 T). A dual echo (in-and-out-of-phase) sequence was used, both with and without spectral presaturation inversion recovery (SPIR) fat suppression (DUAL-SPIR) to visualize BAT, after which all BAT was surgically excised. The BAT volume measurements obtained via (18)FDG-PET/CT and DUAL-SPIR MR were quantitatively compared with the histological findings. All study protocols were reviewed and approved by the local ethics committee.

Results. The BAT mass measurements that were obtained using DUAL-SPIR MR subtraction images correlated better with the histological findings (P = 0.017, R = 0.89) than did the measurements obtained using (18)FDG-PET/CT (P = 0.78, R = 0.15), regardless of the BAT metabolic activation state. Additionally, the basic feasibility of the DUAL-SPIR method was demonstrated in three human pilot subjects.

Conclusions. This study demonstrates the potential for MRI to reliably detect and quantify BAT in vivo. MRI can provide information beyond that provided by (18)FDG-PET imaging, and its ability to detect BAT is independent of its metabolic activation state. Additionally, MRI is a low-cost alternative that does not require radiation. (C) 2013 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)1189-1198
Number of pages10
JournalMETABOLISM-CLINICAL AND EXPERIMENTAL
Volume62
Issue number8
Publication statusPublished - Aug-2013

    Keywords

  • Cold exposure, (18)FDG-PET, Rat, ADULT HUMANS, OUTDOOR TEMPERATURE, 4.7 TESLA, FAT, HIBERNOMA, IDENTIFICATION, WATER, PREVALENCE, OBESITY, MEN

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