Mass spectrometric analysis of PTM dynamics using stable isotope labeled metabolic precursors in cell culturevan Pijkeren, A., Bischoff, R. & Kwiatkowski, M., 7-Dec-2019, In : Analyst. 144, 23, p. 6812-6833 22 p.
Research output: Contribution to journal › Review article › Academic › peer-review
Biological organisms represent highly dynamic systems, which are continually exposed to environmental factors and always strive to restore steady-state homeostasis. Posttranslational modifications are key regulators with which biological systems respond to external stimuli. To understand how homeostasis is restored, it is important to study the kinetics of posttranslational modifications. In this review we discuss proteomic approaches using stable isotope labeled metabolic precursors to study dynamics of posttranslational modifications in cell culture.
|Number of pages||22|
|Early online date||29-Oct-2019|
|Publication status||Published - 7-Dec-2019|
- ACETYL-COA SYNTHETASE, ULTRAVIOLET-LASER DESORPTION, O-GLCNAC TRANSFERASE, QUANTIFYING IN-VIVO, LYSINE ACETYLATION, HISTONE ACETYLATION, ARGININE DIMETHYLATION, PROTEOLYTIC CLEAVAGE, PROTEIN METHYLATION, HIGH-SENSITIVITY