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Low Methoxyl Pectin Protects against Autoimmune Diabetes and Associated Caecal Dysfunction

Wu, C., Pan, L-L., Luo, Y., Niu, W., Fang, X., Liang, W., Li, J., Li, H., Pan, X., Yang, G., Chen, W., Zhang, H., Lakey, J. R. T., Agerberth, B., de Vos, P. & Sun, J., Nov-2019, In : Molecular Nutrition & Food Research. 63, 21, 8 p., e1900307.

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  • Low Methoxyl Pectin Protects against Autoimmune Diabetes and Associated Caecal Dysfunction

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DOI

  • Chengfei Wu
  • Li-Long Pan
  • Yang Luo
  • Wenying Niu
  • Xin Fang
  • Wenjie Liang
  • Jiahong Li
  • Hongli Li
  • Xiaohua Pan
  • Guilian Yang
  • Wei Chen
  • Hao Zhang
  • Jonathan R. T. Lakey
  • Birgitta Agerberth
  • Paul de Vos
  • Jia Sun

SCOPE: This study aims to examine the protective effects of specific low-methoxyl pectin (LMP) on the development of type 1 diabetes (T1D).

METHODS AND RESULTS: Female non-obese diabetic (NOD) mice were weaned onto either control or 5% LMP supplemented diets for up to 22 weeks of age. T1D incidence, gut barrier function, and pancreatic-gut immune responses were analyzed. LMP supplementation significantly dampened the onset of T1D in NOD mice. LMP supplementation induced caecal homeostasis, as indicated by the increasing SCFAs production, higher expression of tight junction proteins claudin 1, zonula occludens-2 in caecum. Furthermore, LMP-mediated caecal homeostasis impacted gut-pancreatic immunity, as evidenced by increased regulatory T cell population, modulated inflammatory cytokine expression, and suppressed NOD like receptor protein 3 (NLRP3) inflammasome activation in both caecum and pancreas.

CONCLUSION: Our data demonstrate that LMP limits T1D development by inducing caecal homeostasis to shape pancreatic immune environment, providing a scientific basis for using LMP as a novel functional supplementation to intervene T1D. This article is protected by copyright. All rights reserved.

Original languageEnglish
Article numbere1900307
Number of pages8
JournalMolecular Nutrition & Food Research
Volume63
Issue number21
Early online date18-Aug-2019
Publication statusPublished - Nov-2019

    Keywords

  • autoimmune diabetes, caecal homeostasis, immunomodulation, low-methoxyl pectin, CHAIN FATTY-ACIDS, INTESTINAL BARRIER FUNCTION, GUT PERMEABILITY, DIETARY FIBER, TYPE-1, MICROBIOTA, BUTYRATE, CONTRIBUTES, METABOLITES, PREBIOTICS

ID: 94742245