Loop grafting of Bacillus subtilis lipase A: Inversion of enantioselectivityBoersma, Y. L., Pijning, T., Bosma, M., van der Sloot, A. M., da Silva Godinho, L., Dröge, M., Winter, R. T., van Pouderoyen, G., Dijkstra, B. W. & Quax, W., 25-Aug-2008, In : Chemistry & Biology. 15, 8, p. 782-789 8 p.
Research output: Contribution to journal › Article › Academic › peer-review
Lipases are successfully applied in enantioselective biocatalysis. Most lipases contain a lid domain controlling access to the active site, but Bacillus subtilis Lipase A (LipA) is a notable exception: its active site is solvent exposed. To improve the enantioselectivity of LipA in the kinetic resolution of 1,2-O-isopropylidene-sn-glycerol (IPG) esters, we replaced a loop near the active-site entrance by longer loops originating from Fusarium solani cutinase and Penicillium purpurogenum acetylxylan esterase, thereby aiming to increase the interaction surface for the substrate. The resulting loop hybrids showed enantioselectivities inverted toward the desired enantiomer of IPG. The acetylxylan esterase-derived variant showed an inversion in enantiomeric excess (ee) from -12.9% to +6.0%, whereas the cutinase-derived variant was improved to an ee of +26.5%. The enantioselectivity of the cutinase-derived variant was further improved by directed evolution to an ee of +57.4%.
|Number of pages||8|
|Journal||Chemistry & Biology|
|Publication status||Published - 25-Aug-2008|
- FUSARIUM-SOLANI CUTINASE, DIRECTED EVOLUTION, PROTEIN-STRUCTURE, INDUSTRIAL BIOCATALYSIS, CRYSTAL-STRUCTURE, ENZYME, SPECIFICITY, SEQUENCE, SELECTION