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Long-term environmental modifications affect BDNF concentrations in rat hippocampus, but not in serum

Giacobbo, B. L., de Freitas, B. S., Vedovelli, K., Schlemmer, L. M., Pires, V. N., Antoniazzi, V., dos Santos, C. D. S., Paludo, L., Borges, J. V., de Lima, D. B., Schroder, N., de Vries, E. F. J. & Bromberg, E., 17-Oct-2019, In : Behavioural Brain Research. 372, 9 p., 111965.

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  • Long-term environmental modifications affect BDNF concentrations in rat hippocampus, but not in serum

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DOI

  • Bruno Lima Giacobbo
  • Betania Souza de Freitas
  • Kelem Vedovelli
  • Livia Machado Schlemmer
  • Vivian Naziaseno Pires
  • Vinicius Antoniazzi
  • Cristophod de Souza dos Santos
  • Leticia Paludo
  • Juliano Viana Borges
  • Daiane Borba de Lima
  • Nadja Schroder
  • Erik F. J. de Vries
  • Elke Bromberg

The role of mBDNF on the beneficial effects of cognitive stimulation on the brain remains controversial, as well as the potential of peripheral mBDNF as a biomarker of environmental effects on its central status. We investigated the effect of different environmental conditions on recognition memory, proBDNF, mBDNF and synaptophysin levels in the hippocampus, and on mBDNF levels in blood. Male Wistar rats (6 and 17 months-old) were assigned to cognitively enriched (EE), standard (SE) and impoverished (IE) environmental conditions for twelve weeks. Novel object recognition was performed at week 10. When the animals were 9 and 20-months old, hippocampus was collected for mBDNF, proBDNF and synaptophysin analysis; serum was analyzed for mBDNF levels. The cognitively EE improved recognition memory, resulted in a trend to increased hippocampal mBDNF and augmented synaptophysin levels. Accordingly, hippocampal mBDNF, proBDNF and synaptophysin were significantly higher in EE than IE animals. Hippocampal mBDNF was positively correlated to proBDNF, cellular and behavioral plasticity markers. No effect of age was seen on the studied variables. Moreover, no significant effects of EE or IE on serum mBDNF were observed. Serum mBDNF also failed to correlate with hippocampal mBDNF, proBDNF and with the cellular and behavioral plasticity markers. These findings indicate that mBDNF is involved in neuronal and behavioral plasticity mechanisms induced by cognitively enriched environments, and that peripheral mBDNF may not always be a reliable biomarker of the effects of environmental settings on central mBDNF and plasticity, which is of special interest from a translational research perspective.

Original languageEnglish
Article number111965
Number of pages9
JournalBehavioural Brain Research
Volume372
Publication statusPublished - 17-Oct-2019

    Keywords

  • BDNF, Environmental enrichment, Hippocampus, serum, Social isolation, proBDNF, DEPRESSION-LIKE BEHAVIORS, NEUROTROPHIC FACTOR BDNF, RECOGNITION MEMORY, SOCIAL-ISOLATION, DENTATE GYRUS, OBJECT RECOGNITION, BRAIN, ENRICHMENT, PROBDNF, EXPRESSION

ID: 94895127