Long-lasting pro-inflammatory suppression of microglia by LPS-preconditioning is mediated by RelB-dependent epigenetic silencingSchaafsma, W., Zhang, X., van Zomeren, K. C., Jacobs, S., Georgieva, P. B., Wolf, S. A., Kettenmann, H., Janova, H., Saiepour, N., Hanisch, U. -K., Meerlo, P., van den Elsen, P. J., Brouwer, N., Boddeke, H. W. G. M. & Eggen, B. J. L., Aug-2015, In : Brain, Behavior, and Immunity. 48, p. 205-221 17 p.
Research output: Contribution to journal › Article › Academic › peer-review
Microglia, the innate immune cells of the central nervous system (CNS), react to endotoxins like bacterial lipopolysaccharides (LPS) with a pronounced inflammatory response. To avoid excess damage to the CNS, the microglia inflammatory response needs to be tightly regulated. Here we report that a single LPS challenge results in a prolonged blunted pro-inflammatory response to a subsequent LPS stimulation, both in primary microglia cultures (100 ng/ml) and in vivo after intraperitoneal (0.25 and 1 mg/kg) or intracere-broventricular (5 mu g) LPS administration. Chromatin immunoprecipitation (ChIP) experiments with primary microglia and microglia acutely isolated from mice showed that LPS preconditioning was accompanied by a reduction in active histone modifications AcH3 and H3K4me3 in the promoters of the IL-10 and TNF-alpha genes. Furthermore, LPS preconditioning resulted in an increase in the amount of repressive histone modification H3K9me2 in the IL-1 beta promoter. ChIP and knock-down experiments showed that NF-kappa B subunit RelB was bound to the IL-1 beta promoter in preconditioned microglia and that RelB is required for the attenuated LPS response. In addition to a suppressed pro-inflammatory response, preconditioned primary microglia displayed enhanced phagocytic activity, increased outward potassium currents and nitric oxide production in response to a second LPS challenge. In vivo, a single i.p. LPS injection resulted in reduced performance in a spatial learning task 4 weeks later, indicating that a single inflammatory episode affected memory formation in these mice. Summarizing, we show that LPS-preconditioned microglia acquire an epigenetically regulated, immune-suppressed phenotype, possibly to prevent excessive damage to the central nervous system in case of recurrent (peripheral) inflammation. (C) 2015 Elsevier Inc. All rights reserved.
|Number of pages||17|
|Journal||Brain, Behavior, and Immunity|
|Publication status||Published - Aug-2015|
- Microglia, Innate immunity, Endotoxin tolerance, Epigenetic silencing, ENDOTOXIN TOLERANCE, CHROMATIN MODIFICATIONS, COGNITIVE IMPAIRMENT, RECEPTOR ACTIVATION, CELL-DEATH, TNF-ALPHA, IN-VIVO, HISTONE, DISTINCT, PHAGOCYTOSIS