Publication

Long telomeres are preferentially extended during recombination-mediated telomere maintenance

Chang, M., Dittmar, J. C. & Rothstein, R., Apr-2011, In : Nature Structural & Molecular Biology. 18, 4, p. 451-456 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

Most human somatic cells do not express telomerase. Consequently, with each cell division their telomeres progressively shorten until replicative senescence is induced. Around 15% of human cancers maintain their telomeres using telomerase-independent, recombination-based mechanisms that are collectively termed 'alternative lengthening of telomeres' (ALT). In the yeast Saccharomyces cerevisiae, ALT cells are referred to as 'survivors'. One type of survivor (type II) resembles human ALT cells in that both are defined by the amplification of telomeric repeats. We analyzed recombination-mediated telomere extension events at individual telomeres in telomerase-negative yeast during the formation of type II survivors and found that long telomeres were preferentially extended. Furthermore, senescent cells with long telomeres were more efficient at bypassing senescence by the type II pathway. We speculate that telomere length may be important in determining whether cancer cells use telomerase or ALT to bypass replicative senescence.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalNature Structural & Molecular Biology
Volume18
Issue number4
Publication statusPublished - Apr-2011
Externally publishedYes

    Keywords

  • SACCHAROMYCES-CEREVISIAE TELOMERES, BREAK-INDUCED REPLICATION, HOMOLOGOUS RECOMBINATION, ESCHERICHIA-COLI, SUBSTRATE LENGTH, MAMMALIAN-CELLS, YEAST TELOMERES, ABSENCE, ELONGATION, SENESCENCE

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