Publication

LmrR-mediated gene regulation of multidrug resistance in Lactococcus lactis

Agustiandari, H., Peeters, E., de Wit, J. G., Charlier, D. & Driessen, A. J. M., May-2011, In : Microbiology-Sgm. 157, 5, p. 1519-1530 12 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Agustiandari, H., Peeters, E., de Wit, J. G., Charlier, D., & Driessen, A. J. M. (2011). LmrR-mediated gene regulation of multidrug resistance in Lactococcus lactis. Microbiology-Sgm, 157(5), 1519-1530. https://doi.org/10.1099/mic.0.048025-0

Author

Agustiandari, Herfita ; Peeters, Eveline ; de Wit, Janny G. ; Charlier, Daniel ; Driessen, Arnold J. M. / LmrR-mediated gene regulation of multidrug resistance in Lactococcus lactis. In: Microbiology-Sgm. 2011 ; Vol. 157, No. 5. pp. 1519-1530.

Harvard

Agustiandari, H, Peeters, E, de Wit, JG, Charlier, D & Driessen, AJM 2011, 'LmrR-mediated gene regulation of multidrug resistance in Lactococcus lactis', Microbiology-Sgm, vol. 157, no. 5, pp. 1519-1530. https://doi.org/10.1099/mic.0.048025-0

Standard

LmrR-mediated gene regulation of multidrug resistance in Lactococcus lactis. / Agustiandari, Herfita; Peeters, Eveline; de Wit, Janny G.; Charlier, Daniel; Driessen, Arnold J. M.

In: Microbiology-Sgm, Vol. 157, No. 5, 05.2011, p. 1519-1530.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Agustiandari H, Peeters E, de Wit JG, Charlier D, Driessen AJM. LmrR-mediated gene regulation of multidrug resistance in Lactococcus lactis. Microbiology-Sgm. 2011 May;157(5):1519-1530. https://doi.org/10.1099/mic.0.048025-0


BibTeX

@article{e0aa05f6b57c49e09e95cef9a3d4c24b,
title = "LmrR-mediated gene regulation of multidrug resistance in Lactococcus lactis",
abstract = "Multidrug resistance (MDR) in Lactococcus lactis is due to the expression of the membrane ATP-binding cassette (ABC) transporter LmrCD. In the absence of drugs, the transcriptional regulator LmrR prevents expression of the ImrCD operon by binding to its operator site. Through an autoregulatory mechanism LmrR also suppresses its own expression. Although the ImrR and ImrCD genes have their own promoters, primer extension analysis showed the presence of a long transcript spanning the entire ImrR ImrCD cluster, in addition to various shorter transcripts harbouring the ImrCD genes only. 'In-gel' Cu-phenanthroline footprinting analysis indicated an extensive interaction between LmrR and the ImrR promoter/operator region. Atomic force microscopy imaging of the binding of LmrR to the control region of ImrR DNA showed severe deformations indicative of DNA wrapping and looping, while LmrR binding to a fragment containing the ImrCD control region induced DNA bending. The results further suggest a drug-dependent regulation mechanism in which the ImrCD genes are co-transcribed with ImrR as a polycistronic messenger. This leads to an LmrR-mediated regulation of ImrCD expression that is exerted from two different locations and by distinct regulatory mechanisms.",
keywords = "SULFOLOBUS-SOLFATARICUS P2, DNA-BINDING PROTEIN, STAPHYLOCOCCUS-AUREUS, TRANSCRIPTIONAL REGULATOR, ESCHERICHIA-COLI, TRANSPORTER LMRCD, FORCE MICROSCOPY, FERULIC ACID, EFFLUX PUMP, SS-LRPB",
author = "Herfita Agustiandari and Eveline Peeters and {de Wit}, {Janny G.} and Daniel Charlier and Driessen, {Arnold J. M.}",
year = "2011",
month = "5",
doi = "10.1099/mic.0.048025-0",
language = "English",
volume = "157",
pages = "1519--1530",
journal = "Microbiology",
issn = "1465-2080",
number = "5",

}

RIS

TY - JOUR

T1 - LmrR-mediated gene regulation of multidrug resistance in Lactococcus lactis

AU - Agustiandari, Herfita

AU - Peeters, Eveline

AU - de Wit, Janny G.

AU - Charlier, Daniel

AU - Driessen, Arnold J. M.

PY - 2011/5

Y1 - 2011/5

N2 - Multidrug resistance (MDR) in Lactococcus lactis is due to the expression of the membrane ATP-binding cassette (ABC) transporter LmrCD. In the absence of drugs, the transcriptional regulator LmrR prevents expression of the ImrCD operon by binding to its operator site. Through an autoregulatory mechanism LmrR also suppresses its own expression. Although the ImrR and ImrCD genes have their own promoters, primer extension analysis showed the presence of a long transcript spanning the entire ImrR ImrCD cluster, in addition to various shorter transcripts harbouring the ImrCD genes only. 'In-gel' Cu-phenanthroline footprinting analysis indicated an extensive interaction between LmrR and the ImrR promoter/operator region. Atomic force microscopy imaging of the binding of LmrR to the control region of ImrR DNA showed severe deformations indicative of DNA wrapping and looping, while LmrR binding to a fragment containing the ImrCD control region induced DNA bending. The results further suggest a drug-dependent regulation mechanism in which the ImrCD genes are co-transcribed with ImrR as a polycistronic messenger. This leads to an LmrR-mediated regulation of ImrCD expression that is exerted from two different locations and by distinct regulatory mechanisms.

AB - Multidrug resistance (MDR) in Lactococcus lactis is due to the expression of the membrane ATP-binding cassette (ABC) transporter LmrCD. In the absence of drugs, the transcriptional regulator LmrR prevents expression of the ImrCD operon by binding to its operator site. Through an autoregulatory mechanism LmrR also suppresses its own expression. Although the ImrR and ImrCD genes have their own promoters, primer extension analysis showed the presence of a long transcript spanning the entire ImrR ImrCD cluster, in addition to various shorter transcripts harbouring the ImrCD genes only. 'In-gel' Cu-phenanthroline footprinting analysis indicated an extensive interaction between LmrR and the ImrR promoter/operator region. Atomic force microscopy imaging of the binding of LmrR to the control region of ImrR DNA showed severe deformations indicative of DNA wrapping and looping, while LmrR binding to a fragment containing the ImrCD control region induced DNA bending. The results further suggest a drug-dependent regulation mechanism in which the ImrCD genes are co-transcribed with ImrR as a polycistronic messenger. This leads to an LmrR-mediated regulation of ImrCD expression that is exerted from two different locations and by distinct regulatory mechanisms.

KW - SULFOLOBUS-SOLFATARICUS P2

KW - DNA-BINDING PROTEIN

KW - STAPHYLOCOCCUS-AUREUS

KW - TRANSCRIPTIONAL REGULATOR

KW - ESCHERICHIA-COLI

KW - TRANSPORTER LMRCD

KW - FORCE MICROSCOPY

KW - FERULIC ACID

KW - EFFLUX PUMP

KW - SS-LRPB

U2 - 10.1099/mic.0.048025-0

DO - 10.1099/mic.0.048025-0

M3 - Article

VL - 157

SP - 1519

EP - 1530

JO - Microbiology

JF - Microbiology

SN - 1465-2080

IS - 5

ER -

ID: 5339795