Publication

Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function

Schuettpelz, L. G., Gopalan, P. K., Giuste, F. O., Romine, M. P., van Os, R. & Link, D. C., 11-Oct-2012, In : Blood. 120, 15, p. 2981-2989 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Schuettpelz, L. G., Gopalan, P. K., Giuste, F. O., Romine, M. P., van Os, R., & Link, D. C. (2012). Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function. Blood, 120(15), 2981-2989. https://doi.org/10.1182/blood-2012-02-409839

Author

Schuettpelz, Laura G. ; Gopalan, Priya K. ; Giuste, Felipe O. ; Romine, Molly P. ; van Os, Ronald ; Link, Daniel C. / Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function. In: Blood. 2012 ; Vol. 120, No. 15. pp. 2981-2989.

Harvard

Schuettpelz, LG, Gopalan, PK, Giuste, FO, Romine, MP, van Os, R & Link, DC 2012, 'Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function', Blood, vol. 120, no. 15, pp. 2981-2989. https://doi.org/10.1182/blood-2012-02-409839

Standard

Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function. / Schuettpelz, Laura G.; Gopalan, Priya K.; Giuste, Felipe O.; Romine, Molly P.; van Os, Ronald; Link, Daniel C.

In: Blood, Vol. 120, No. 15, 11.10.2012, p. 2981-2989.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Schuettpelz LG, Gopalan PK, Giuste FO, Romine MP, van Os R, Link DC. Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function. Blood. 2012 Oct 11;120(15):2981-2989. https://doi.org/10.1182/blood-2012-02-409839


BibTeX

@article{00b978c94d7744e4a240e141df16b0d4,
title = "Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function",
abstract = "Increased expression of Kruppel-like factor 7 (KLF7) is an independent predictor of poor outcome in pediatric acute lymphoblastic leukemia. The contribution of KLF7 to hematopoiesis has not been previously described. Herein, we characterized the effect on murine hematopoiesis of the loss of KLF7 and enforced expression of KLF7. Long-term multilineage engraftment of Klf7(-/-) cells was comparable with control cells, and self-renewal, as assessed by serial transplantation, was not affected. Enforced expression of KLF7 results in a marked suppression of myeloid progenitor cell growth and a loss of short- and long-term repopulating activity. Interestingly, enforced expression of KLF7, although resulting in multilineage growth suppression that extended to hematopoietic stem cells and common lymphoid progenitors, spared T cells and enhanced the survival of early thymocytes. RNA expression profiling of KLF7-overexpressing hematopoietic progenitors identified several potential target genes mediating these effects. Notably, the known KLF7 target Cdkn1a (p21(Cip1/Waf1)) was not induced by KLF7, and loss of CDKN1A does not rescue the repopulating defect. These results suggest that KLF7 is not required for normal hematopoietic stem and progenitor function, but increased expression, as seen in a subset of lymphoid leukemia, inhibits myeloid cell proliferation and promotes early thymocyte survival. (Blood. 2012; 120(15): 2981-2989)",
keywords = "ACUTE LYMPHOBLASTIC-LEUKEMIA, TRANSCRIPTION FACTOR KLF7, CHRONIC LYMPHOCYTIC-LEUKEMIA, POTENTIAL MECHANISM, SENSORY NEURONS, UP-REGULATION, SELF-RENEWAL, EXPRESSION, BFL-1, GENE",
author = "Schuettpelz, {Laura G.} and Gopalan, {Priya K.} and Giuste, {Felipe O.} and Romine, {Molly P.} and {van Os}, Ronald and Link, {Daniel C.}",
year = "2012",
month = "10",
day = "11",
doi = "10.1182/blood-2012-02-409839",
language = "English",
volume = "120",
pages = "2981--2989",
journal = "Blood",
issn = "0006-4971",
publisher = "AMER SOC HEMATOLOGY",
number = "15",

}

RIS

TY - JOUR

T1 - Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function

AU - Schuettpelz, Laura G.

AU - Gopalan, Priya K.

AU - Giuste, Felipe O.

AU - Romine, Molly P.

AU - van Os, Ronald

AU - Link, Daniel C.

PY - 2012/10/11

Y1 - 2012/10/11

N2 - Increased expression of Kruppel-like factor 7 (KLF7) is an independent predictor of poor outcome in pediatric acute lymphoblastic leukemia. The contribution of KLF7 to hematopoiesis has not been previously described. Herein, we characterized the effect on murine hematopoiesis of the loss of KLF7 and enforced expression of KLF7. Long-term multilineage engraftment of Klf7(-/-) cells was comparable with control cells, and self-renewal, as assessed by serial transplantation, was not affected. Enforced expression of KLF7 results in a marked suppression of myeloid progenitor cell growth and a loss of short- and long-term repopulating activity. Interestingly, enforced expression of KLF7, although resulting in multilineage growth suppression that extended to hematopoietic stem cells and common lymphoid progenitors, spared T cells and enhanced the survival of early thymocytes. RNA expression profiling of KLF7-overexpressing hematopoietic progenitors identified several potential target genes mediating these effects. Notably, the known KLF7 target Cdkn1a (p21(Cip1/Waf1)) was not induced by KLF7, and loss of CDKN1A does not rescue the repopulating defect. These results suggest that KLF7 is not required for normal hematopoietic stem and progenitor function, but increased expression, as seen in a subset of lymphoid leukemia, inhibits myeloid cell proliferation and promotes early thymocyte survival. (Blood. 2012; 120(15): 2981-2989)

AB - Increased expression of Kruppel-like factor 7 (KLF7) is an independent predictor of poor outcome in pediatric acute lymphoblastic leukemia. The contribution of KLF7 to hematopoiesis has not been previously described. Herein, we characterized the effect on murine hematopoiesis of the loss of KLF7 and enforced expression of KLF7. Long-term multilineage engraftment of Klf7(-/-) cells was comparable with control cells, and self-renewal, as assessed by serial transplantation, was not affected. Enforced expression of KLF7 results in a marked suppression of myeloid progenitor cell growth and a loss of short- and long-term repopulating activity. Interestingly, enforced expression of KLF7, although resulting in multilineage growth suppression that extended to hematopoietic stem cells and common lymphoid progenitors, spared T cells and enhanced the survival of early thymocytes. RNA expression profiling of KLF7-overexpressing hematopoietic progenitors identified several potential target genes mediating these effects. Notably, the known KLF7 target Cdkn1a (p21(Cip1/Waf1)) was not induced by KLF7, and loss of CDKN1A does not rescue the repopulating defect. These results suggest that KLF7 is not required for normal hematopoietic stem and progenitor function, but increased expression, as seen in a subset of lymphoid leukemia, inhibits myeloid cell proliferation and promotes early thymocyte survival. (Blood. 2012; 120(15): 2981-2989)

KW - ACUTE LYMPHOBLASTIC-LEUKEMIA

KW - TRANSCRIPTION FACTOR KLF7

KW - CHRONIC LYMPHOCYTIC-LEUKEMIA

KW - POTENTIAL MECHANISM

KW - SENSORY NEURONS

KW - UP-REGULATION

KW - SELF-RENEWAL

KW - EXPRESSION

KW - BFL-1

KW - GENE

U2 - 10.1182/blood-2012-02-409839

DO - 10.1182/blood-2012-02-409839

M3 - Article

VL - 120

SP - 2981

EP - 2989

JO - Blood

JF - Blood

SN - 0006-4971

IS - 15

ER -

ID: 5729671