TY - JOUR

T1 - KINETICS OF PERITONEAL B-1A CELLS (CD5 B-CELLS) IN YOUNG-ADULT MICE

AU - DEENEN, GJ

AU - KROESE, FGM

PY - 1993/1

Y1 - 1993/1

N2 - In the mouse, conventional B cells are continuously generated from precursor cells located in the bone marrow (BM), whereas the small subset of B-1 cells (formerly called Ly-1 B cells) constitute a self-replenishing population of cells. Here we studied the kinetics of murine peritoneal B-1a cells (i.e. B-1 cells expressing CD5). The actual number of B-1a cells in the peritoneal cavity that are proliferating, as detected by metaphase arrest and S-phase index, was below detection level, indicating that these cells do not divide significantly at this anatomical location. To establish the life-span of B-1a cells we used long-term administration of 5-bromo-2'-deoxyuridine in combination with three-color immunocytology on cytospin preparations. The renewal rate of peritoneal B-1a cells was 1.3% per day representing a 50% renewal time of 38 days. Splenic B cells and popliteal lymph node B cells (predominantly conventional B cells) showed an almost identical renewal rate of 1.1 % per day. The data show that peripheral B cells from various lymphoid tissues and locations do not differ significantly in their renewal capacity, even though there are differences in their developmental origin.

AB - In the mouse, conventional B cells are continuously generated from precursor cells located in the bone marrow (BM), whereas the small subset of B-1 cells (formerly called Ly-1 B cells) constitute a self-replenishing population of cells. Here we studied the kinetics of murine peritoneal B-1a cells (i.e. B-1 cells expressing CD5). The actual number of B-1a cells in the peritoneal cavity that are proliferating, as detected by metaphase arrest and S-phase index, was below detection level, indicating that these cells do not divide significantly at this anatomical location. To establish the life-span of B-1a cells we used long-term administration of 5-bromo-2'-deoxyuridine in combination with three-color immunocytology on cytospin preparations. The renewal rate of peritoneal B-1a cells was 1.3% per day representing a 50% renewal time of 38 days. Splenic B cells and popliteal lymph node B cells (predominantly conventional B cells) showed an almost identical renewal rate of 1.1 % per day. The data show that peripheral B cells from various lymphoid tissues and locations do not differ significantly in their renewal capacity, even though there are differences in their developmental origin.

KW - B-1 CELLS

KW - KINETICS

KW - IMMUNOCYTOLOGY

KW - BROMODEOXYURIDINE

KW - PERIPHERAL LYMPHOID ORGANS

KW - BONE-MARROW

KW - LY-1 B

KW - POPULATION-KINETICS

KW - MURINE-B

KW - LYMPHOCYTES

KW - MOUSE

KW - BROMODEOXYURIDINE

KW - PROLIFERATION

KW - EXPRESSION

M3 - Article

VL - 23

SP - 12

EP - 16

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 1

ER -