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KINETICS OF B-CELL SUBPOPULATIONS IN PERIPHERAL LYMPHOID-TISSUES - EVIDENCE FOR THE PRESENCE OF PHENOTYPICALLY DISTINCT SHORT-LIVED AND LONG-LIVED B-CELL SUBSETS

DEENEN, GJ. & KROESE, FGM., Jul-1993, In : International immunology. 5, 7, p. 735-741 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

A small proportion of the sIg+ B lymphocytes in peripheral lymphoid organs [22% in spleen and 6% in lymph node (LN)] in rat carries the Thy-1 antigen. These Thy-1 + B cells represent newly formed bone marrow (BM) derived (or immature) B cells. In this study we investigated the kinetic behavior of Thy-1+ and Thy-1- B cells in various lymphoid tissues. The renewal rates of these B cells in young adult rats were determined by continuous administration of 5-bromo-2-deoxyuridine (BrdU) for up to 6 weeks. At several time intervals, Thy-1+ and Thy-1- B cell subpopulations in BM, blood, spleen and popliteal LN were analyzed for the presence of incorporated BrdU, using three-color immunocytology on cytospin preparations. In all tissues studied, the Thy-1+ B cells were rapidly renewed with a rate that varied between 58% (BM) and 22% (LN) per day. Virtually all Thy-1+ B cells were labeled by BrdU within a period of 8 - 16 days, indicating that all these cells are relatively short-lived. By contrast, the replacement of Thy-1- B cells in these tissues was 30 - 40 times lower, and ranged between 2.0 and 0.8% per day. In absolute numbers we estimate that 57 million Thy-1+ B cells are renewed per day in the BM whereas only 10 million Thy-1- B cells are replaced in the pool of long-lived peripheral B cells. This implicates a cell loss of 80% at the transition of the Thy-1+ to the Thy-1- B cell stage. The few B cells that actually become incorporated into the pool of mature B cells are most probably selected on the basis of the specificity of their sIg.

Original languageEnglish
Pages (from-to)735-741
Number of pages7
JournalInternational immunology
Volume5
Issue number7
Publication statusPublished - Jul-1993

    Keywords

  • B-CELLS, 5-BROMO-2-DEOXYURIDINE, IMMUNOCYTOLOGY, KINETICS, THY-1 ANTIGEN, TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE, REACTIVE LYMPHOCYTES-B, RAT BONE-MARROW, POPULATION-KINETICS, MICE, MOUSE, ANTIGEN, BROMODEOXYURIDINE, IMMUNOGLOBULIN, EXPRESSION

ID: 6355423