Publication

Isolation of Mouse salivary gland stem cells

Pringle, S., Nanduri, L., van der Zwaag, M., van Os, R. & Coppes, R., 8-Feb-2011, In : JoVE (Journal of Visualized Experiments). 48, February, p. e2482 3 p., 2011 Feb 8;(48). pii.

Research output: Contribution to journalArticleAcademic

APA

Pringle, S., Nanduri, L., van der Zwaag, M., van Os, R., & Coppes, R. (2011). Isolation of Mouse salivary gland stem cells. JoVE (Journal of Visualized Experiments), 48(February), e2482. [2011 Feb 8;(48). pii]. https://doi.org/10.3791/2484

Author

Pringle, Sarah ; Nanduri, Lalitha ; van der Zwaag, Marianne ; van Os, Ronald ; Coppes, Rob. / Isolation of Mouse salivary gland stem cells. In: JoVE (Journal of Visualized Experiments). 2011 ; Vol. 48, No. February. pp. e2482.

Harvard

Pringle, S, Nanduri, L, van der Zwaag, M, van Os, R & Coppes, R 2011, 'Isolation of Mouse salivary gland stem cells', JoVE (Journal of Visualized Experiments), vol. 48, no. February, 2011 Feb 8;(48). pii, pp. e2482. https://doi.org/10.3791/2484

Standard

Isolation of Mouse salivary gland stem cells. / Pringle, Sarah; Nanduri, Lalitha; van der Zwaag, Marianne; van Os, Ronald; Coppes, Rob.

In: JoVE (Journal of Visualized Experiments), Vol. 48, No. February, 2011 Feb 8;(48). pii, 08.02.2011, p. e2482.

Research output: Contribution to journalArticleAcademic

Vancouver

Pringle S, Nanduri L, van der Zwaag M, van Os R, Coppes R. Isolation of Mouse salivary gland stem cells. JoVE (Journal of Visualized Experiments). 2011 Feb 8;48(February):e2482. 2011 Feb 8;(48). pii. https://doi.org/10.3791/2484


BibTeX

@article{b5af3662b7064d72b2c7a0a5ebe1b0d1,
title = "Isolation of Mouse salivary gland stem cells",
abstract = "Mature salivary glands of both human and mouse origin comprise a minimum of five cell types, each of which facilitates the production andexcretion of saliva into the oral cavity. Serous and mucous acinar cells are the protein and mucous producing factories of the gland respectively,and represent the origin of saliva production. Once synthesised, the various enzymatic and other proteinaceous components of saliva aresecreted through a series of ductal cells bearing epithelial-type morphology, until the eventual expulsion of the saliva through one major duct intothe cavity of the mouth. The composition of saliva is also modified by the ductal cells during this process.In the manifestation of diseases such as Sj{\"o}gren's syndrome, and in some clinical situations such as radiotherapy treatment for head and neckcancers, saliva production by the glands is dramatically reduced 1,2. The resulting xerostomia, a subjective feeling of dry mouth, affects not onlythe ability of the patient to swallow and speak, but also encourages the development of dental caries and can be socially debilitating for thesufferer.The restoration of saliva production in the above-mentioned clinical conditions therefore represents an unmet clinical need, and as such severalstudies have demonstrated the regenerative capacity of the salivary glands 3-5. Further to the isolation of stem cell-like populations of cells fromvarious tissues within the mouse and human bodies 6-8, we have shown using the described method that stem cells isolated from mouse salivaryglands can be used to rescue saliva production in irradiated salivary glands 9,10. This discovery paves the way for the development of stem cellbasedtherapies for the treatment of xerostomic conditions in humans, and also for the exploration of the salivary gland as a microenvironmentcontaining cells with multipotent self-renewing capabilities.",
author = "Sarah Pringle and Lalitha Nanduri and {van der Zwaag}, Marianne and {van Os}, Ronald and Rob Coppes",
year = "2011",
month = "2",
day = "8",
doi = "10.3791/2484",
language = "English",
volume = "48",
pages = "e2482",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "JOURNAL OF VISUALIZED EXPERIMENTS",
number = "February",

}

RIS

TY - JOUR

T1 - Isolation of Mouse salivary gland stem cells

AU - Pringle, Sarah

AU - Nanduri, Lalitha

AU - van der Zwaag, Marianne

AU - van Os, Ronald

AU - Coppes, Rob

PY - 2011/2/8

Y1 - 2011/2/8

N2 - Mature salivary glands of both human and mouse origin comprise a minimum of five cell types, each of which facilitates the production andexcretion of saliva into the oral cavity. Serous and mucous acinar cells are the protein and mucous producing factories of the gland respectively,and represent the origin of saliva production. Once synthesised, the various enzymatic and other proteinaceous components of saliva aresecreted through a series of ductal cells bearing epithelial-type morphology, until the eventual expulsion of the saliva through one major duct intothe cavity of the mouth. The composition of saliva is also modified by the ductal cells during this process.In the manifestation of diseases such as Sjögren's syndrome, and in some clinical situations such as radiotherapy treatment for head and neckcancers, saliva production by the glands is dramatically reduced 1,2. The resulting xerostomia, a subjective feeling of dry mouth, affects not onlythe ability of the patient to swallow and speak, but also encourages the development of dental caries and can be socially debilitating for thesufferer.The restoration of saliva production in the above-mentioned clinical conditions therefore represents an unmet clinical need, and as such severalstudies have demonstrated the regenerative capacity of the salivary glands 3-5. Further to the isolation of stem cell-like populations of cells fromvarious tissues within the mouse and human bodies 6-8, we have shown using the described method that stem cells isolated from mouse salivaryglands can be used to rescue saliva production in irradiated salivary glands 9,10. This discovery paves the way for the development of stem cellbasedtherapies for the treatment of xerostomic conditions in humans, and also for the exploration of the salivary gland as a microenvironmentcontaining cells with multipotent self-renewing capabilities.

AB - Mature salivary glands of both human and mouse origin comprise a minimum of five cell types, each of which facilitates the production andexcretion of saliva into the oral cavity. Serous and mucous acinar cells are the protein and mucous producing factories of the gland respectively,and represent the origin of saliva production. Once synthesised, the various enzymatic and other proteinaceous components of saliva aresecreted through a series of ductal cells bearing epithelial-type morphology, until the eventual expulsion of the saliva through one major duct intothe cavity of the mouth. The composition of saliva is also modified by the ductal cells during this process.In the manifestation of diseases such as Sjögren's syndrome, and in some clinical situations such as radiotherapy treatment for head and neckcancers, saliva production by the glands is dramatically reduced 1,2. The resulting xerostomia, a subjective feeling of dry mouth, affects not onlythe ability of the patient to swallow and speak, but also encourages the development of dental caries and can be socially debilitating for thesufferer.The restoration of saliva production in the above-mentioned clinical conditions therefore represents an unmet clinical need, and as such severalstudies have demonstrated the regenerative capacity of the salivary glands 3-5. Further to the isolation of stem cell-like populations of cells fromvarious tissues within the mouse and human bodies 6-8, we have shown using the described method that stem cells isolated from mouse salivaryglands can be used to rescue saliva production in irradiated salivary glands 9,10. This discovery paves the way for the development of stem cellbasedtherapies for the treatment of xerostomic conditions in humans, and also for the exploration of the salivary gland as a microenvironmentcontaining cells with multipotent self-renewing capabilities.

U2 - 10.3791/2484

DO - 10.3791/2484

M3 - Article

VL - 48

SP - e2482

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - February

M1 - 2011 Feb 8;(48). pii

ER -

ID: 14046421