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Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol

Duque, A. F., Hasan, S. A., Bessa, V. S., Carvalho, M. F., Samin, G., Janssen, D. B. & Castro, P. M. L., Jul-2012, In : Applied Microbiology and Biotechnology. 95, 2, p. 511-520 10 p.

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DOI

  • Anouk F. Duque
  • Syed A. Hasan
  • Vania S. Bessa
  • Maria F. Carvalho
  • Ghufrana Samin
  • Dick B. Janssen
  • Paula M. L. Castro

A pure bacterial culture able to utilize 2-fluorophenol (2-FP) as sole carbon and energy source was isolated by selective enrichment from sediments collected from a contaminated site in Northern Portugal. 16S rRNA gene analysis showed that the organism (strain FP1) belongs to the genus Rhodococcus. When grown aerobically on 2-FP, growth kinetics of strain FP1 followed the Luong model. An inhibitory effect of increasing 2-FP concentrations was observed with no growth occurring at 2-FP levels higher than ca. 4 mM. Rhodococcus strain FP1 was able to degrade a range of other organofluorine compounds, including 2-fluorobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-fluorophenol, 4-fluorophenol, 3-fluorocatechol, and 4-fluorocatechol, as well as chlorinated compounds such as 2-chlorophenol and 4-chlorophenol. Experiments with cell-free extracts and partially purified enzymes indicated that the first step of 2-fluorophenol metabolism was conversion to 3-fluorocatechol, suggesting an unusual pathway for fluoroaromatic metabolism. To our knowledge, this is the first time that utilization of 2-FP as a growth substrate by a pure bacterial culture is reported.

Original languageEnglish
Pages (from-to)511-520
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume95
Issue number2
Publication statusPublished - Jul-2012

    Keywords

  • 2-Fluorophenol (2-FP), Rhodococcus sp., Biodegradation, Luong model, Monooxygenase, CHLOROPHENOL 4-MONOOXYGENASE TFTD, OPACUS 1CP, 4-CHLOROPHENOL DEGRADATION, BACTERIAL-DEGRADATION, OXIDOREDUCTASE TFTC, FLUOROBENZOIC ACID, GENUS RHODOCOCCUS, PSEUDOMONAS SP, BIODEGRADATION, DEHALOGENATION

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