Publication

Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis

van Sleen, Y., Wang, Q., van der Geest, K. S. M., Westra, J., Abdulahad, W. H., Heeringa, P., Boots, A. M. H. & Brouwer, E., 26-Jul-2017, In : Scientific Reports. 7, 11 p., 6553.

Research output: Contribution to journalArticleAcademicpeer-review

APA

van Sleen, Y., Wang, Q., van der Geest, K. S. M., Westra, J., Abdulahad, W. H., Heeringa, P., ... Brouwer, E. (2017). Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis. Scientific Reports, 7, [6553]. https://doi.org/10.1038/s41598-017-06826-4

Author

van Sleen, Yannick ; Wang, Qi ; van der Geest, Kornelis S. M. ; Westra, Johanna ; Abdulahad, Wayel H. ; Heeringa, Peter ; Boots, Annemieke M. H. ; Brouwer, Elisabeth. / Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis. In: Scientific Reports. 2017 ; Vol. 7.

Harvard

van Sleen, Y, Wang, Q, van der Geest, KSM, Westra, J, Abdulahad, WH, Heeringa, P, Boots, AMH & Brouwer, E 2017, 'Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis' Scientific Reports, vol. 7, 6553. https://doi.org/10.1038/s41598-017-06826-4

Standard

Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis. / van Sleen, Yannick; Wang, Qi; van der Geest, Kornelis S. M.; Westra, Johanna; Abdulahad, Wayel H.; Heeringa, Peter; Boots, Annemieke M. H.; Brouwer, Elisabeth.

In: Scientific Reports, Vol. 7, 6553, 26.07.2017.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

van Sleen Y, Wang Q, van der Geest KSM, Westra J, Abdulahad WH, Heeringa P et al. Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis. Scientific Reports. 2017 Jul 26;7. 6553. https://doi.org/10.1038/s41598-017-06826-4


BibTeX

@article{24df8b62a6314d308d99b0e24e25195b,
title = "Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis",
abstract = "Monocytes/macrophages are critical in systemic and local inflammation in giant cell arteritis (GCA) and possibly in clinically overlapping polymyalgia rheumatica (PMR). Therefore, we aimed to understand the contribution of monocyte subsets and the CX3CR1-CX3CL1 and CCR2-CCL2 migratory pathways, to the pathology of GCA. Peripheral blood monocytes were enumerated in samples from newly-diagnosed, untreated GCA and PMR patients and after prednisone-induced remission. The distribution of classical (CD14(bright)CD16(neg)) and the more pro-inflammatory, intermediate (CD14(bright)CD16+) and non-classical (CD14(dim)CD16+) monocyte subsets was analysed by flow cytometry. The phenotype of macrophages in temporal artery biopsies (TABs) from GCA patients was studied by immunohistochemistry and immunofluorescence. A clear monocytosis was seen in newly diagnosed GCA and PMR patients caused by elevated numbers of classical monocytes. Prednisone treatment suppressed numbers of non-classical monocytes. Both chemokine CX3CL1 and CCL2 were highly expressed in the TAB. Most macrophages in the TAB of GCA patients expressed non-classical monocyte markers CD16 and CX3CR1 whereas co-localisation of CD16 with classical monocyte marker CCR2 was infrequent. In conclusion, we report an altered distribution of monocyte subsets in both GCA and PMR patients. The majority of macrophages in TABs of GCA patients were CD68 + CD16 + CX3CR1 + CCR2- and thereby resembled the phenotype of non-classical monocytes.",
keywords = "RHEUMATOID-ARTHRITIS PATIENTS, INFLAMMATORY-BOWEL-DISEASE, POLYMYALGIA-RHEUMATICA, CD16(+) MONOCYTES, SINUSOIDAL ENDOTHELIUM, ANCA AUTOANTIGENS, BLOOD MONOCYTES, DENDRITIC CELLS, BONE-MARROW, FC-GAMMA",
author = "{van Sleen}, Yannick and Qi Wang and {van der Geest}, {Kornelis S. M.} and Johanna Westra and Abdulahad, {Wayel H.} and Peter Heeringa and Boots, {Annemieke M. H.} and Elisabeth Brouwer",
year = "2017",
month = "7",
day = "26",
doi = "10.1038/s41598-017-06826-4",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis

AU - van Sleen, Yannick

AU - Wang, Qi

AU - van der Geest, Kornelis S. M.

AU - Westra, Johanna

AU - Abdulahad, Wayel H.

AU - Heeringa, Peter

AU - Boots, Annemieke M. H.

AU - Brouwer, Elisabeth

PY - 2017/7/26

Y1 - 2017/7/26

N2 - Monocytes/macrophages are critical in systemic and local inflammation in giant cell arteritis (GCA) and possibly in clinically overlapping polymyalgia rheumatica (PMR). Therefore, we aimed to understand the contribution of monocyte subsets and the CX3CR1-CX3CL1 and CCR2-CCL2 migratory pathways, to the pathology of GCA. Peripheral blood monocytes were enumerated in samples from newly-diagnosed, untreated GCA and PMR patients and after prednisone-induced remission. The distribution of classical (CD14(bright)CD16(neg)) and the more pro-inflammatory, intermediate (CD14(bright)CD16+) and non-classical (CD14(dim)CD16+) monocyte subsets was analysed by flow cytometry. The phenotype of macrophages in temporal artery biopsies (TABs) from GCA patients was studied by immunohistochemistry and immunofluorescence. A clear monocytosis was seen in newly diagnosed GCA and PMR patients caused by elevated numbers of classical monocytes. Prednisone treatment suppressed numbers of non-classical monocytes. Both chemokine CX3CL1 and CCL2 were highly expressed in the TAB. Most macrophages in the TAB of GCA patients expressed non-classical monocyte markers CD16 and CX3CR1 whereas co-localisation of CD16 with classical monocyte marker CCR2 was infrequent. In conclusion, we report an altered distribution of monocyte subsets in both GCA and PMR patients. The majority of macrophages in TABs of GCA patients were CD68 + CD16 + CX3CR1 + CCR2- and thereby resembled the phenotype of non-classical monocytes.

AB - Monocytes/macrophages are critical in systemic and local inflammation in giant cell arteritis (GCA) and possibly in clinically overlapping polymyalgia rheumatica (PMR). Therefore, we aimed to understand the contribution of monocyte subsets and the CX3CR1-CX3CL1 and CCR2-CCL2 migratory pathways, to the pathology of GCA. Peripheral blood monocytes were enumerated in samples from newly-diagnosed, untreated GCA and PMR patients and after prednisone-induced remission. The distribution of classical (CD14(bright)CD16(neg)) and the more pro-inflammatory, intermediate (CD14(bright)CD16+) and non-classical (CD14(dim)CD16+) monocyte subsets was analysed by flow cytometry. The phenotype of macrophages in temporal artery biopsies (TABs) from GCA patients was studied by immunohistochemistry and immunofluorescence. A clear monocytosis was seen in newly diagnosed GCA and PMR patients caused by elevated numbers of classical monocytes. Prednisone treatment suppressed numbers of non-classical monocytes. Both chemokine CX3CL1 and CCL2 were highly expressed in the TAB. Most macrophages in the TAB of GCA patients expressed non-classical monocyte markers CD16 and CX3CR1 whereas co-localisation of CD16 with classical monocyte marker CCR2 was infrequent. In conclusion, we report an altered distribution of monocyte subsets in both GCA and PMR patients. The majority of macrophages in TABs of GCA patients were CD68 + CD16 + CX3CR1 + CCR2- and thereby resembled the phenotype of non-classical monocytes.

KW - RHEUMATOID-ARTHRITIS PATIENTS

KW - INFLAMMATORY-BOWEL-DISEASE

KW - POLYMYALGIA-RHEUMATICA

KW - CD16(+) MONOCYTES

KW - SINUSOIDAL ENDOTHELIUM

KW - ANCA AUTOANTIGENS

KW - BLOOD MONOCYTES

KW - DENDRITIC CELLS

KW - BONE-MARROW

KW - FC-GAMMA

U2 - 10.1038/s41598-017-06826-4

DO - 10.1038/s41598-017-06826-4

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 6553

ER -

ID: 46160256