Publication

Intestinal microbiota and anastomotic leakage of stapled colorectal anastomoses: a pilot study

van Praagh, J. B., de Goffau, M. C., Bakker, I. S., Harmsen, H. J. M., Olinga, P. & Havenga, K., Jun-2016, In : Surgical endoscopy and other interventional techniques. 30, 6, p. 2259-2265 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

Background Anastomotic leakage (AL) after colorectal surgery is a severe complication, resulting in morbidity, reinterventions, prolonged hospital stay and, in some cases, death. Some technical and patient-related aetiological factors of AL are well established. In many cases, however, none of these factors seem to explain the occurrence of AL. Recent studies suggest that the intestinal microbiome plays a role in wound healing, diabetes and Crohn's disease. The aim of this study was to compare the intestinal microbiota of patients who developed AL with matched patients with healed colorectal anastomoses.

Methods We investigated the microbiome in the doughnuts collected from 16 patients participating in the C-seal trial. We selected eight patients who developed AL requiring reintervention and eight matched controls without AL. We analysed the bacterial 16S rDNA of both groups with MiSeq sequencing.

Results The abundance of Lachnospiraceae is statistically higher (P = 0.001) in patient group who did develop AL, while microbial diversity levels were higher in the group who did not develop AL (P = 0.037). Body mass index (BMI) was also positively associated with the abundance of the Lachnospiraceae family (P = 0.022).

Conclusion A correlation between the bacterial family Lachnospiraceae, low microbial diversity and anastomotic leakage, possibly in association with the BMI, was found. The relative abundance of the Lachnospiraceae family is possibly explained by the higher abundance of mucin-degrading Ruminococci within that family in AL cases (P = 0.011) as is similarly the case in IBD.

Original languageEnglish
Pages (from-to)2259-2265
Number of pages7
JournalSurgical endoscopy and other interventional techniques
Volume30
Issue number6
Publication statusPublished - Jun-2016
EventAnnual Meeting of the Society-of-American-Gastrointestinal-and-Endoscopic-Surgeons (SAGES) - Nashville, Tunisia
Duration: 15-Apr-201518-Apr-2015

Event

Annual Meeting of the Society-of-American-Gastrointestinal-and-Endoscopic-Surgeons (SAGES)

15/04/201518/04/2015

Nashville, Tunisia

Event: Conference

    Keywords

  • Anastomotic leakage, Intestinal microbiome, Colorectal surgery, Stapled anastomosis, DNA sequencing, Complications, GUT MICROBIOTA, BUTYRATE, SURGERY, BACTERIA, OBESITY, INFLAMMATION, ENVIRONMENT, DEHISCENCE, RESECTION, CHILDREN

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