Publication

Interaction of Streptococcus mutans YidC1 and YidC2 with Translating and Nontranslating Ribosomes

Wu, Z. C., de Keyzer, J., Berrelkamp-Lahpor, G. A. & Driessen, A. J. M., Oct-2013, In : Journal of Bacteriology. 195, 19, p. 4545-4551 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Wu, Z. C., de Keyzer, J., Berrelkamp-Lahpor, G. A., & Driessen, A. J. M. (2013). Interaction of Streptococcus mutans YidC1 and YidC2 with Translating and Nontranslating Ribosomes. Journal of Bacteriology, 195(19), 4545-4551. https://doi.org/10.1128/JB.00792-13

Author

Wu, Zht Cheng ; de Keyzer, Jeanine ; Berrelkamp-Lahpor, Greetje A. ; Driessen, Arnold J. M. / Interaction of Streptococcus mutans YidC1 and YidC2 with Translating and Nontranslating Ribosomes. In: Journal of Bacteriology. 2013 ; Vol. 195, No. 19. pp. 4545-4551.

Harvard

Wu, ZC, de Keyzer, J, Berrelkamp-Lahpor, GA & Driessen, AJM 2013, 'Interaction of Streptococcus mutans YidC1 and YidC2 with Translating and Nontranslating Ribosomes', Journal of Bacteriology, vol. 195, no. 19, pp. 4545-4551. https://doi.org/10.1128/JB.00792-13

Standard

Interaction of Streptococcus mutans YidC1 and YidC2 with Translating and Nontranslating Ribosomes. / Wu, Zht Cheng; de Keyzer, Jeanine; Berrelkamp-Lahpor, Greetje A.; Driessen, Arnold J. M.

In: Journal of Bacteriology, Vol. 195, No. 19, 10.2013, p. 4545-4551.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Wu ZC, de Keyzer J, Berrelkamp-Lahpor GA, Driessen AJM. Interaction of Streptococcus mutans YidC1 and YidC2 with Translating and Nontranslating Ribosomes. Journal of Bacteriology. 2013 Oct;195(19):4545-4551. https://doi.org/10.1128/JB.00792-13


BibTeX

@article{7052897242034a18a2ab648bc4e530b4,
title = "Interaction of Streptococcus mutans YidC1 and YidC2 with Translating and Nontranslating Ribosomes",
abstract = "The YidC/OxaI/Alb3 family of membrane proteins is involved in the biogenesis of integral membrane proteins in bacteria, mitochondria, and chloroplasts. Gram-positive bacteria often contain multiple YidC paralogs that can be subdivided into two major classes, namely, YidC1 and YidC2. The Streptococcus mutans YidC1 and YidC2 proteins possess C-terminal tails that differ in charges (+9 and +14) and lengths (33 and 61 amino acids). The longer YidC2 C terminus bears a resemblance to the C-terminal ribosome-binding domain of the mitochondrial OxaI protein and, in contrast to the shorter YidC1 C terminus, can mediate the interaction with mitochondrial ribosomes. These observations have led to the suggestion that YidC1 and YidC2 differ in their abilities to interact with ribosomes. However, the interaction with bacterial translating ribosomes has never been addressed. Here we demonstrate that Escherichia coli ribosomes are able to interact with both YidC1 and YidC2. The interaction is stimulated by the presence of a nascent membrane protein substrate and abolished upon deletion of the C-terminal tail, which also abrogates the YidC-dependent membrane insertion of subunit c of the F1F0-ATPase into the membrane. It is concluded that both YidC1 and YidC2 interact with ribosomes, suggesting that the modes of membrane insertion by these membrane insertases are similar.",
keywords = "NASCENT CHAIN COMPLEXES, ESCHERICHIA-COLI YIDC, MITOCHONDRIAL RIBOSOME, BACILLUS-SUBTILIS, PROTEIN SECRETION, SPOIIIJ GENE, FAMILY, EXPRESSION, BACTERIAL, PATHWAY",
author = "Wu, {Zht Cheng} and {de Keyzer}, Jeanine and Berrelkamp-Lahpor, {Greetje A.} and Driessen, {Arnold J. M.}",
year = "2013",
month = oct,
doi = "10.1128/JB.00792-13",
language = "English",
volume = "195",
pages = "4545--4551",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "AMER SOC MICROBIOLOGY",
number = "19",

}

RIS

TY - JOUR

T1 - Interaction of Streptococcus mutans YidC1 and YidC2 with Translating and Nontranslating Ribosomes

AU - Wu, Zht Cheng

AU - de Keyzer, Jeanine

AU - Berrelkamp-Lahpor, Greetje A.

AU - Driessen, Arnold J. M.

PY - 2013/10

Y1 - 2013/10

N2 - The YidC/OxaI/Alb3 family of membrane proteins is involved in the biogenesis of integral membrane proteins in bacteria, mitochondria, and chloroplasts. Gram-positive bacteria often contain multiple YidC paralogs that can be subdivided into two major classes, namely, YidC1 and YidC2. The Streptococcus mutans YidC1 and YidC2 proteins possess C-terminal tails that differ in charges (+9 and +14) and lengths (33 and 61 amino acids). The longer YidC2 C terminus bears a resemblance to the C-terminal ribosome-binding domain of the mitochondrial OxaI protein and, in contrast to the shorter YidC1 C terminus, can mediate the interaction with mitochondrial ribosomes. These observations have led to the suggestion that YidC1 and YidC2 differ in their abilities to interact with ribosomes. However, the interaction with bacterial translating ribosomes has never been addressed. Here we demonstrate that Escherichia coli ribosomes are able to interact with both YidC1 and YidC2. The interaction is stimulated by the presence of a nascent membrane protein substrate and abolished upon deletion of the C-terminal tail, which also abrogates the YidC-dependent membrane insertion of subunit c of the F1F0-ATPase into the membrane. It is concluded that both YidC1 and YidC2 interact with ribosomes, suggesting that the modes of membrane insertion by these membrane insertases are similar.

AB - The YidC/OxaI/Alb3 family of membrane proteins is involved in the biogenesis of integral membrane proteins in bacteria, mitochondria, and chloroplasts. Gram-positive bacteria often contain multiple YidC paralogs that can be subdivided into two major classes, namely, YidC1 and YidC2. The Streptococcus mutans YidC1 and YidC2 proteins possess C-terminal tails that differ in charges (+9 and +14) and lengths (33 and 61 amino acids). The longer YidC2 C terminus bears a resemblance to the C-terminal ribosome-binding domain of the mitochondrial OxaI protein and, in contrast to the shorter YidC1 C terminus, can mediate the interaction with mitochondrial ribosomes. These observations have led to the suggestion that YidC1 and YidC2 differ in their abilities to interact with ribosomes. However, the interaction with bacterial translating ribosomes has never been addressed. Here we demonstrate that Escherichia coli ribosomes are able to interact with both YidC1 and YidC2. The interaction is stimulated by the presence of a nascent membrane protein substrate and abolished upon deletion of the C-terminal tail, which also abrogates the YidC-dependent membrane insertion of subunit c of the F1F0-ATPase into the membrane. It is concluded that both YidC1 and YidC2 interact with ribosomes, suggesting that the modes of membrane insertion by these membrane insertases are similar.

KW - NASCENT CHAIN COMPLEXES

KW - ESCHERICHIA-COLI YIDC

KW - MITOCHONDRIAL RIBOSOME

KW - BACILLUS-SUBTILIS

KW - PROTEIN SECRETION

KW - SPOIIIJ GENE

KW - FAMILY

KW - EXPRESSION

KW - BACTERIAL

KW - PATHWAY

U2 - 10.1128/JB.00792-13

DO - 10.1128/JB.00792-13

M3 - Article

VL - 195

SP - 4545

EP - 4551

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 19

ER -

ID: 5954838