Publication

Interaction of differently designed immunoliposomes with colon cancer cells and Kupffer cells. An in vitro comparison

Koning, GA., Morselt, HWM., Gorter, A., Allen, TM., Zalipsky, S., Scherphof, GL. & Kamps, JAAM., Aug-2003, In : Pharmaceutical Research. 20, 8, p. 1249-1257 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • GA Koning
  • HWM Morselt
  • A Gorter
  • TM Allen
  • S Zalipsky
  • GL Scherphof
  • JAAM Kamps

Purpose. Evaluate the effectiveness of distal-end coupling of a tumor-specific antibody to liposomal polyethylene glycol (PEG) chains to improve target binding and reduce interference by macrophage uptake.

Methods. Monoclonal antibody CC52, specific for CC531 rat colon carcinoma, was coupled to the bilayer of PEG-liposomes (type I) or to the distal end of bilayer-anchored PEG-chains (type II). Uptake of both (radiolabeled) liposome types by CC531 cells and rat liver macrophages was determined.

Results. With increasing antibody density, both immunoliposome types showed increased binding to target cells, but type II liposomes displayed better target recognition than type I. Uptake by macrophages increased with antibody density for both liposome types. Lowest uptake by macrophages was found for type II liposomes at low antibody densities. Unexpectedly, not only for type I but also for type II liposomes, in which the antibody is coupled via its Fc moiety, uptake by macrophages was inhibited by aggregated IgG, indicating involvement of Fc receptors. Also polyinosinic acid, an inhibitor of scavenger receptors, reduced uptake of type II liposomes.

Conclusion. Although distal end coupling of antibodies to bilayer-anchored PEG chains in liposomes through the Fc moiety enhances target cell binding, it does not prevent the recognition by Fc receptors on macrophages.

Original languageEnglish
Pages (from-to)1249-1257
Number of pages9
JournalPharmaceutical Research
Volume20
Issue number8
Publication statusPublished - Aug-2003

    Keywords

  • immunoliposomes, poly(ethylene glycol), colon cancer, Kupffer cells, tumor specific antibody, drug-targeting, STERICALLY STABILIZED LIPOSOMES, CIRCULATION TIME, MONOCLONAL-ANTIBODIES, SOLID TUMORS, RECEPTOR, VIVO, ADENOCARCINOMA, ATTACHMENT, METASTASES, INVITRO

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