Publication

Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes

Kijlstra, J. D., Hu, D., Mittal, N., Kausel, E., van der Meer, P., Garakani, A. & Domian, I. J., 8-Dec-2015, In : Stem Cell Reports. 5, 6, p. 1226-1238 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Kijlstra, J. D., Hu, D., Mittal, N., Kausel, E., van der Meer, P., Garakani, A., & Domian, I. J. (2015). Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes. Stem Cell Reports, 5(6), 1226-1238. https://doi.org/10.1016/j.stemcr.2015.10.017

Author

Kijlstra, Jan David ; Hu, Dongjian ; Mittal, Nikhil ; Kausel, Eduardo ; van der Meer, Peter ; Garakani, Arman ; Domian, Ibrahim J. / Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes. In: Stem Cell Reports. 2015 ; Vol. 5, No. 6. pp. 1226-1238.

Harvard

Kijlstra, JD, Hu, D, Mittal, N, Kausel, E, van der Meer, P, Garakani, A & Domian, IJ 2015, 'Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes', Stem Cell Reports, vol. 5, no. 6, pp. 1226-1238. https://doi.org/10.1016/j.stemcr.2015.10.017

Standard

Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes. / Kijlstra, Jan David; Hu, Dongjian; Mittal, Nikhil; Kausel, Eduardo; van der Meer, Peter; Garakani, Arman; Domian, Ibrahim J.

In: Stem Cell Reports, Vol. 5, No. 6, 08.12.2015, p. 1226-1238.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Kijlstra JD, Hu D, Mittal N, Kausel E, van der Meer P, Garakani A et al. Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes. Stem Cell Reports. 2015 Dec 8;5(6):1226-1238. https://doi.org/10.1016/j.stemcr.2015.10.017


BibTeX

@article{1ba884229dac4d088a0dc077904c9785,
title = "Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes",
abstract = "The quantitative analysis of cardiomyocyte function is essential for stem cell-based approaches for the in vitro study of human cardiac physiology and pathophysiology. We present a method to comprehensively assess the function of single human pluripotent stem cell-derived cardiomyocyte (hPSC-CMs) through simultaneous quantitative analysis of contraction kinetics, force generation, and electrical activity. We demonstrate that statistical analysis of movies of contracting hPSC-CMs can be used to quantify changes in cellular morphology over time and compute contractile kinetics. Using a biomechanical model that incorporates substrate stiffness, we calculate cardiomyocyte force generation at single-cell resolution and validate this approach with conventional traction force microscopy. The addition of fluorescent calcium indicators or membrane potential dyes allows the simultaneous analysis of contractility and calcium handling or action potential morphology. Accordingly, our approach has the potential for broad application in the study of cardiac disease, drug discovery, and cardiotoxicity screening.",
keywords = "MYOCYTE SHAPE, CARDIOTOXICITY, HEART, MODULATION, DYNAMICS, PLATFORM, VOLTAGE",
author = "Kijlstra, {Jan David} and Dongjian Hu and Nikhil Mittal and Eduardo Kausel and {van der Meer}, Peter and Arman Garakani and Domian, {Ibrahim J.}",
year = "2015",
month = "12",
day = "8",
doi = "10.1016/j.stemcr.2015.10.017",
language = "English",
volume = "5",
pages = "1226--1238",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "CELL PRESS",
number = "6",

}

RIS

TY - JOUR

T1 - Integrated Analysis of Contractile Kinetics, Force Generation, and Electrical Activity in Single Human Stem Cell-Derived Cardiomyocytes

AU - Kijlstra, Jan David

AU - Hu, Dongjian

AU - Mittal, Nikhil

AU - Kausel, Eduardo

AU - van der Meer, Peter

AU - Garakani, Arman

AU - Domian, Ibrahim J.

PY - 2015/12/8

Y1 - 2015/12/8

N2 - The quantitative analysis of cardiomyocyte function is essential for stem cell-based approaches for the in vitro study of human cardiac physiology and pathophysiology. We present a method to comprehensively assess the function of single human pluripotent stem cell-derived cardiomyocyte (hPSC-CMs) through simultaneous quantitative analysis of contraction kinetics, force generation, and electrical activity. We demonstrate that statistical analysis of movies of contracting hPSC-CMs can be used to quantify changes in cellular morphology over time and compute contractile kinetics. Using a biomechanical model that incorporates substrate stiffness, we calculate cardiomyocyte force generation at single-cell resolution and validate this approach with conventional traction force microscopy. The addition of fluorescent calcium indicators or membrane potential dyes allows the simultaneous analysis of contractility and calcium handling or action potential morphology. Accordingly, our approach has the potential for broad application in the study of cardiac disease, drug discovery, and cardiotoxicity screening.

AB - The quantitative analysis of cardiomyocyte function is essential for stem cell-based approaches for the in vitro study of human cardiac physiology and pathophysiology. We present a method to comprehensively assess the function of single human pluripotent stem cell-derived cardiomyocyte (hPSC-CMs) through simultaneous quantitative analysis of contraction kinetics, force generation, and electrical activity. We demonstrate that statistical analysis of movies of contracting hPSC-CMs can be used to quantify changes in cellular morphology over time and compute contractile kinetics. Using a biomechanical model that incorporates substrate stiffness, we calculate cardiomyocyte force generation at single-cell resolution and validate this approach with conventional traction force microscopy. The addition of fluorescent calcium indicators or membrane potential dyes allows the simultaneous analysis of contractility and calcium handling or action potential morphology. Accordingly, our approach has the potential for broad application in the study of cardiac disease, drug discovery, and cardiotoxicity screening.

KW - MYOCYTE SHAPE

KW - CARDIOTOXICITY

KW - HEART

KW - MODULATION

KW - DYNAMICS

KW - PLATFORM

KW - VOLTAGE

U2 - 10.1016/j.stemcr.2015.10.017

DO - 10.1016/j.stemcr.2015.10.017

M3 - Article

C2 - 26626178

VL - 5

SP - 1226

EP - 1238

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 6

ER -

ID: 28325275