Publication

Insulin-like growth factor-binding protein 6 inhibits survival and differentiation of rat oligodendrocyte precursor cells

Kuhl, NM., Hoekstra, D., De Vries, H. & De Keyser, J., Nov-2003, In : Glia. 44, 2, p. 91-101 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

Insulin-like growth factor 1 (IGF-1) is a growth and survival factor for oligodendrocyte lineage cells and promotes myelination. We demonstrate that IGF-binding protein 6 (IGFBP-6) is expressed and localized to the Golgi complex in rat oligodendrocyte precursor (O2A) cells. IGFBP-6 mRNA showed a developmentally regulated expression pattern, displaying a transient decrease during early development, and enhanced levels upon cell maturation. IGFBP-6 mRNA expression could be reduced by addition of basic fibroblast growth factor and progesterone while estrogen increased IGFBP-6 mRNA. IGF-1, platelet-derived growth factor, and insulin had no effect. When added exogenously, IGFBP-6 reduced O2A cell survival in the absence of IGF-1 and inhibited IGF-1-stimulated survival in a partially IGF-1-dependent and partially IGF-1-independent fashion. In addition, IGFBP-6 reduced the IGF-stimulated expression of two myelin proteins, CNPase and MAG. Taken together, the data show that IGFBP-6 is a new negative effector of oligodendrocyte survival and differentiation. (C) 2003 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)91-101
Number of pages11
JournalGlia
Volume44
Issue number2
Publication statusPublished - Nov-2003

    Keywords

  • O2A cells, survival, myelin proteins, mRNA, des(1-3)-IGF-1, GROWTH-FACTOR-I, FACTOR-BINDING-PROTEIN, EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS, CENTRAL-NERVOUS-SYSTEM, FACTOR (IGF)-BINDING PROTEINS, HUMAN NEUROBLASTOMA-CELLS, IGF-I, CEREBROSPINAL-FLUID, SELECTIVE AFFINITY, GENE-EXPRESSION

ID: 4136551