Inhibition of the miR-155 target NIAM phenocopies the growth promoting effect of miR-155 in B-cell lymphomaKluiver, J., Slezak-Prochazka, I., de Jong, D., Smigielska, K., Kortman, G., Winkle, M., Rutgers, B., Koerts, J., Visser, L., Diepstra, A., Kroesen, B-J. & van den Berg, A., 19-Jan-2016, In : Oncotarget. 7, 3, p. 2391-2400 10 p.
Research output: Contribution to journal › Article › Academic › peer-review
Several studies have indicated an important role for miR-155 in the pathogenesis of B-cell lymphoma. Highly elevated levels of miR-155 were indeed observed in most B-cell lymphomas with the exception of Burkitt lymphoma (BL). However, the molecular mechanisms that underlie the oncogenic role of miR-155 in B-cell lymphoma are not well understood. To identify the miR-155 targets relevant for B-cell lymphoma, we performed RNA immunoprecipitation of Argonaute 2 in Hodgkin lymphoma (HL) cells upon miR-155 inhibition and in BL cells upon ectopic expression of miR-155. We identified 54 miR-155-specific target genes in BL cells and confirmed miR-155 targeting of DET1, NIAM, TRIM32, HOMEZ, PSIP1 and JARID2. Five of these targets are also regulated by endogenous miR-155 in HL cells. Both overexpression of miR-155 and inhibition of expression of the novel miR-155 target gene NIAM increased proliferation of BL cells. In primary B-cell lymphoma NIAM-positive cases have significant lower levels of miR-155 as compared to NIAM-negative cases, suggesting that NIAM is also regulated by miR-155 in primary B-cell lymphoma. Thus, our data indicate an oncogenic role for miR-155 in B-cell lymphoma which involves targeting the tumor suppressor NIAM.
|Number of pages||10|
|Publication status||Published - 19-Jan-2016|
- B-cell lymphoma, NIAM, Ago2-IP, miR-155, TBRG1, INDUCED CYTIDINE DEAMINASE, NUCLEAR INTERACTOR, HODGKIN LYMPHOMA, BURKITT-LYMPHOMA, GENE-EXPRESSION, MICRORNA-155, ACTIVATION, MDM2, ARF, MYC
Inhibition of the miR-155 target NIAM phenocopies the growth promoting effect of miR-155 in B-cell lymphoma [L1236]