Publication

Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano

Wudarski, J., Ustyantsev, K., Glazenburg, L. & Berezikov, E., 12-Feb-2019, In : Zoological Letters. 5, 1, 10 p., 7.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Wudarski, J., Ustyantsev, K., Glazenburg, L., & Berezikov, E. (2019). Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano. Zoological Letters, 5(1), [7]. https://doi.org/10.1186/s40851-019-0122-6

Author

Wudarski, Jakub ; Ustyantsev, Kirill ; Glazenburg, Lisa ; Berezikov, Eugene. / Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano. In: Zoological Letters. 2019 ; Vol. 5, No. 1.

Harvard

Wudarski, J, Ustyantsev, K, Glazenburg, L & Berezikov, E 2019, 'Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano', Zoological Letters, vol. 5, no. 1, 7. https://doi.org/10.1186/s40851-019-0122-6

Standard

Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano. / Wudarski, Jakub; Ustyantsev, Kirill; Glazenburg, Lisa; Berezikov, Eugene.

In: Zoological Letters, Vol. 5, No. 1, 7, 12.02.2019.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Wudarski J, Ustyantsev K, Glazenburg L, Berezikov E. Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano. Zoological Letters. 2019 Feb 12;5(1). 7. https://doi.org/10.1186/s40851-019-0122-6


BibTeX

@article{ac6960f5bcac49e7b83ecbf2c5eca4c1,
title = "Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano",
abstract = "Background: The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is unique among flatworm models. However, due to its relatively recent introduction in research, many aspects of this animal's biology remain unknown. One such question is the influence of culture temperature on Macrostomum biology. Results: We systematically investigated how different culture temperatures affect development time, reproduction rate, regeneration, heat shock response, and gene knockdown efficiency by RNA interference (RNAi) in M. lignano. We used marker transgenic lines to accurately measure the regeneration endpoint, and to establish the stress response threshold for temperature shock. We found that compared to the culture temperature of 20 °C commonly used for M. lignano, temperatures of 25 °C-30 °C substantially increase the speed of development and regeneration, lead to faster manifestation of RNAi phenotypes, and increase reproduction rate without detectable negative consequences for the animal, while temperatures above 30 °C elicit a heat shock response. Conclusions: We show that altering temperature conditions can be used to reduce the time required to establish M. lignano cultures, perform RNAi experiments, store important lines, and optimize microinjection procedures for transgenesis. These findings will help to optimize the design of experiments in M. lignano, and thus facilitate future research using this model organism.",
keywords = "Fertility, Flatworms, Heat shock response, Macrostomum lignano, Regeneration, Temperature",
author = "Jakub Wudarski and Kirill Ustyantsev and Lisa Glazenburg and Eugene Berezikov",
year = "2019",
month = feb,
day = "12",
doi = "10.1186/s40851-019-0122-6",
language = "English",
volume = "5",
journal = "Zoological Letters",
issn = "2056-306X",
number = "1",

}

RIS

TY - JOUR

T1 - Influence of temperature on development, reproduction and regeneration in the flatworm model organism, Macrostomum lignano

AU - Wudarski, Jakub

AU - Ustyantsev, Kirill

AU - Glazenburg, Lisa

AU - Berezikov, Eugene

PY - 2019/2/12

Y1 - 2019/2/12

N2 - Background: The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is unique among flatworm models. However, due to its relatively recent introduction in research, many aspects of this animal's biology remain unknown. One such question is the influence of culture temperature on Macrostomum biology. Results: We systematically investigated how different culture temperatures affect development time, reproduction rate, regeneration, heat shock response, and gene knockdown efficiency by RNA interference (RNAi) in M. lignano. We used marker transgenic lines to accurately measure the regeneration endpoint, and to establish the stress response threshold for temperature shock. We found that compared to the culture temperature of 20 °C commonly used for M. lignano, temperatures of 25 °C-30 °C substantially increase the speed of development and regeneration, lead to faster manifestation of RNAi phenotypes, and increase reproduction rate without detectable negative consequences for the animal, while temperatures above 30 °C elicit a heat shock response. Conclusions: We show that altering temperature conditions can be used to reduce the time required to establish M. lignano cultures, perform RNAi experiments, store important lines, and optimize microinjection procedures for transgenesis. These findings will help to optimize the design of experiments in M. lignano, and thus facilitate future research using this model organism.

AB - Background: The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is unique among flatworm models. However, due to its relatively recent introduction in research, many aspects of this animal's biology remain unknown. One such question is the influence of culture temperature on Macrostomum biology. Results: We systematically investigated how different culture temperatures affect development time, reproduction rate, regeneration, heat shock response, and gene knockdown efficiency by RNA interference (RNAi) in M. lignano. We used marker transgenic lines to accurately measure the regeneration endpoint, and to establish the stress response threshold for temperature shock. We found that compared to the culture temperature of 20 °C commonly used for M. lignano, temperatures of 25 °C-30 °C substantially increase the speed of development and regeneration, lead to faster manifestation of RNAi phenotypes, and increase reproduction rate without detectable negative consequences for the animal, while temperatures above 30 °C elicit a heat shock response. Conclusions: We show that altering temperature conditions can be used to reduce the time required to establish M. lignano cultures, perform RNAi experiments, store important lines, and optimize microinjection procedures for transgenesis. These findings will help to optimize the design of experiments in M. lignano, and thus facilitate future research using this model organism.

KW - Fertility

KW - Flatworms

KW - Heat shock response

KW - Macrostomum lignano

KW - Regeneration

KW - Temperature

U2 - 10.1186/s40851-019-0122-6

DO - 10.1186/s40851-019-0122-6

M3 - Article

AN - SCOPUS:85061513994

VL - 5

JO - Zoological Letters

JF - Zoological Letters

SN - 2056-306X

IS - 1

M1 - 7

ER -

ID: 76802378