Publication

Influence of docosahexaenoic acid in vitro on intracellular adriamycin concentration in lymphocytes and human adriamycin-sensitive and -resistant small-cell lung cancer cell lines, and on cytotoxicity in the tumor cell lines

Zijlstra, J. G., de Vries, E. G., Muskiet, F. A., Martini, I. A., Timmer-Bosscha, H. & Mulder, N. H., 15-Dec-1987, In : International Journal of Cancer. 40, 6, p. 850-856 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Zijlstra, J. G., de Vries, E. G., Muskiet, F. A., Martini, I. A., Timmer-Bosscha, H., & Mulder, N. H. (1987). Influence of docosahexaenoic acid in vitro on intracellular adriamycin concentration in lymphocytes and human adriamycin-sensitive and -resistant small-cell lung cancer cell lines, and on cytotoxicity in the tumor cell lines. International Journal of Cancer, 40(6), 850-856. https://doi.org/10.1002/ijc.2910400625

Author

Zijlstra, J G ; de Vries, E G ; Muskiet, F A ; Martini, I A ; Timmer-Bosscha, H ; Mulder, N H. / Influence of docosahexaenoic acid in vitro on intracellular adriamycin concentration in lymphocytes and human adriamycin-sensitive and -resistant small-cell lung cancer cell lines, and on cytotoxicity in the tumor cell lines. In: International Journal of Cancer. 1987 ; Vol. 40, No. 6. pp. 850-856.

Harvard

Zijlstra, JG, de Vries, EG, Muskiet, FA, Martini, IA, Timmer-Bosscha, H & Mulder, NH 1987, 'Influence of docosahexaenoic acid in vitro on intracellular adriamycin concentration in lymphocytes and human adriamycin-sensitive and -resistant small-cell lung cancer cell lines, and on cytotoxicity in the tumor cell lines', International Journal of Cancer, vol. 40, no. 6, pp. 850-856. https://doi.org/10.1002/ijc.2910400625

Standard

Influence of docosahexaenoic acid in vitro on intracellular adriamycin concentration in lymphocytes and human adriamycin-sensitive and -resistant small-cell lung cancer cell lines, and on cytotoxicity in the tumor cell lines. / Zijlstra, J G; de Vries, E G; Muskiet, F A; Martini, I A; Timmer-Bosscha, H; Mulder, N H.

In: International Journal of Cancer, Vol. 40, No. 6, 15.12.1987, p. 850-856.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Zijlstra JG, de Vries EG, Muskiet FA, Martini IA, Timmer-Bosscha H, Mulder NH. Influence of docosahexaenoic acid in vitro on intracellular adriamycin concentration in lymphocytes and human adriamycin-sensitive and -resistant small-cell lung cancer cell lines, and on cytotoxicity in the tumor cell lines. International Journal of Cancer. 1987 Dec 15;40(6):850-856. https://doi.org/10.1002/ijc.2910400625


BibTeX

@article{947c66c1838748989a5e5efac14b9596,
title = "Influence of docosahexaenoic acid in vitro on intracellular adriamycin concentration in lymphocytes and human adriamycin-sensitive and -resistant small-cell lung cancer cell lines, and on cytotoxicity in the tumor cell lines",
abstract = "An increase in the therapeutic effects of cancer chemotherapeutic agents and circumvention of drug resistance in cancer cells might result from an increase in the intracellular drug level. Alteration of the lipid domain of the cell membrane can result in a higher intracellular drug level. This alteration was achieved in human lymphocytes and in human adriamycin (ADR)-sensitive and -resistant small-cell lung carcinoma cells in vitro by incubation with docosahexaenoic acid (22:6). Incorporation of the fatty acid in cellular phospholipids was measured by gas chromatographic analysis. A significant increase of 22:6 could be reached without loss of viability in all 3 cell types. Incorporation was demonstrated notably in the phosphatidyl choline, phosphatidyl ethanolamine and phosphatidylserine, and was most pronounced in the phosphatidyl choline of the ADR-resistant line. After a 1-hr incubation with ADR, a 10-30% increase in intracellular adriamycin concentration was found in all 3 cell types previously incubated for 4 days with 22:6. After 1 hr incubation with ADR there was no increase in cytotoxicity in the sensitive cell line when measured by soft agar clonogenic assay and a partial reversal (52 to 14) of resistance factor (ratio of drug doses to produce 50% growth inhibition) in the resistant cell line. Increasing the time of ADR exposure from 1 to 4 hr further reduced the resistance factor to 8.6.",
keywords = "Biological Transport, Carcinoma, Small Cell, Cell Division, Cell Survival, Docosahexaenoic Acids, Doxorubicin, Drug Resistance, Humans, Intracellular Fluid, Lipids, Lung Neoplasms, Lymphocytes, Tumor Cells, Cultured",
author = "Zijlstra, {J G} and {de Vries}, {E G} and Muskiet, {F A} and Martini, {I A} and H Timmer-Bosscha and Mulder, {N H}",
year = "1987",
month = dec,
day = "15",
doi = "10.1002/ijc.2910400625",
language = "English",
volume = "40",
pages = "850--856",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley",
number = "6",

}

RIS

TY - JOUR

T1 - Influence of docosahexaenoic acid in vitro on intracellular adriamycin concentration in lymphocytes and human adriamycin-sensitive and -resistant small-cell lung cancer cell lines, and on cytotoxicity in the tumor cell lines

AU - Zijlstra, J G

AU - de Vries, E G

AU - Muskiet, F A

AU - Martini, I A

AU - Timmer-Bosscha, H

AU - Mulder, N H

PY - 1987/12/15

Y1 - 1987/12/15

N2 - An increase in the therapeutic effects of cancer chemotherapeutic agents and circumvention of drug resistance in cancer cells might result from an increase in the intracellular drug level. Alteration of the lipid domain of the cell membrane can result in a higher intracellular drug level. This alteration was achieved in human lymphocytes and in human adriamycin (ADR)-sensitive and -resistant small-cell lung carcinoma cells in vitro by incubation with docosahexaenoic acid (22:6). Incorporation of the fatty acid in cellular phospholipids was measured by gas chromatographic analysis. A significant increase of 22:6 could be reached without loss of viability in all 3 cell types. Incorporation was demonstrated notably in the phosphatidyl choline, phosphatidyl ethanolamine and phosphatidylserine, and was most pronounced in the phosphatidyl choline of the ADR-resistant line. After a 1-hr incubation with ADR, a 10-30% increase in intracellular adriamycin concentration was found in all 3 cell types previously incubated for 4 days with 22:6. After 1 hr incubation with ADR there was no increase in cytotoxicity in the sensitive cell line when measured by soft agar clonogenic assay and a partial reversal (52 to 14) of resistance factor (ratio of drug doses to produce 50% growth inhibition) in the resistant cell line. Increasing the time of ADR exposure from 1 to 4 hr further reduced the resistance factor to 8.6.

AB - An increase in the therapeutic effects of cancer chemotherapeutic agents and circumvention of drug resistance in cancer cells might result from an increase in the intracellular drug level. Alteration of the lipid domain of the cell membrane can result in a higher intracellular drug level. This alteration was achieved in human lymphocytes and in human adriamycin (ADR)-sensitive and -resistant small-cell lung carcinoma cells in vitro by incubation with docosahexaenoic acid (22:6). Incorporation of the fatty acid in cellular phospholipids was measured by gas chromatographic analysis. A significant increase of 22:6 could be reached without loss of viability in all 3 cell types. Incorporation was demonstrated notably in the phosphatidyl choline, phosphatidyl ethanolamine and phosphatidylserine, and was most pronounced in the phosphatidyl choline of the ADR-resistant line. After a 1-hr incubation with ADR, a 10-30% increase in intracellular adriamycin concentration was found in all 3 cell types previously incubated for 4 days with 22:6. After 1 hr incubation with ADR there was no increase in cytotoxicity in the sensitive cell line when measured by soft agar clonogenic assay and a partial reversal (52 to 14) of resistance factor (ratio of drug doses to produce 50% growth inhibition) in the resistant cell line. Increasing the time of ADR exposure from 1 to 4 hr further reduced the resistance factor to 8.6.

KW - Biological Transport

KW - Carcinoma, Small Cell

KW - Cell Division

KW - Cell Survival

KW - Docosahexaenoic Acids

KW - Doxorubicin

KW - Drug Resistance

KW - Humans

KW - Intracellular Fluid

KW - Lipids

KW - Lung Neoplasms

KW - Lymphocytes

KW - Tumor Cells, Cultured

U2 - 10.1002/ijc.2910400625

DO - 10.1002/ijc.2910400625

M3 - Article

C2 - 2826341

VL - 40

SP - 850

EP - 856

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 6

ER -

ID: 23242077