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Infliximab Does Not Promote the Presence of Collagenolytic Bacteria in a Mouse Model of Colorectal Anastomosis

Gaines, S., Hyoju, S., Williamson, A. J., van Praagh, J. B., Zaborina, O., Rubin, D. T., Alverdy, J. C., Shogan, B. D. & Hyman, N., 2-Jan-2020, In : Journal of Gastrointestinal Surgery. 6 p.

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  • Infliximab Does Not Promote the Presence of Collagenolytic Bacteria in a Mouse Model of Colorectal Anastomosis

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DOI

  • Sara Gaines
  • Sanjiv Hyoju
  • Ashley J. Williamson
  • Jasper B. van Praagh
  • Olga Zaborina
  • David T. Rubin
  • John C. Alverdy
  • Benjamin D. Shogan
  • Neil Hyman

BACKGROUND: Previous work from our group has suggested a pivotal role for collagenolytic bacteria in the development of anastomotic complications. Tumor necrosis factor antagonists are a mainstay of treatment for patients with inflammatory bowel disease. The reported impact of these agents on key surgical outcomes such as anastomotic leak has been inconsistent. The objective of this study is to assess the impact of infliximab on the anastomotic microbiome in a mouse model of colon resection.

DESIGN: BALB/c mice underwent colon resection with primary anastomosis. Mice were randomly assigned to receive either an intraperitoneal dose of saline (control) or 10 mg/kg of infliximab for 8 weeks prior to surgery. On postoperative day 7, the animals were sacrificed. Anastomotic tissues were analyzed by histology with TUNNEL staining as a marker of epithelial apoptosis. In order to assess compositional and functional changes of the local microbiome, anastomotic tissues were further analyzed by 16S rRNA V4 region sequencing and for the presence of collagenolytic strains that may impair anastomotic healing. The main outcome measures were microbiome community structure and the presence of collagenolytic bacteria.

RESULTS: Infliximab-treated mice demonstrated an increase in epithelial apoptosis, consistent with the expected drug effect. Although infliximab modified the perianastomotic microbiome, no increase in the presence of collagenolytic bacteria was observed.

CONCLUSIONS: Infliximab did not promote the emergence of collagenolytic bacteria or demonstrably impair anastomotic healing in a mouse model of colon resection and anastomosis.

Original languageEnglish
Number of pages6
JournalJournal of Gastrointestinal Surgery
Publication statusPublished - 2-Jan-2020

    Keywords

  • Infliximab, Anastomotic leak, Microbiome, ANTITUMOR NECROSIS FACTOR, INFLAMMATORY-BOWEL-DISEASE, ANTI-TNF TREATMENT, GUT MICROBIOME, CROHNS-DISEASE, POSTOPERATIVE COMPLICATIONS, THERAPY, IMPACT, OUTCOMES, AGENTS

ID: 119061085