Publication

In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs

Simon, L. M., Morandi, E., Luganini, A., Gribaudo, G., Martinez-Sobrido, L., Turner, D. H., Oliviero, S. & Incarnato, D., 26-Jul-2019, In : Nucleic Acids Research. 47, 13, p. 7003-7017 15 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Simon, L. M., Morandi, E., Luganini, A., Gribaudo, G., Martinez-Sobrido, L., Turner, D. H., Oliviero, S., & Incarnato, D. (2019). In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs. Nucleic Acids Research, 47(13), 7003-7017. https://doi.org/10.1093/nar/gkz318

Author

Simon, Lisa Marie ; Morandi, Edoardo ; Luganini, Anna ; Gribaudo, Giorgio ; Martinez-Sobrido, Luis ; Turner, Douglas H. ; Oliviero, Salvatore ; Incarnato, Danny. / In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs. In: Nucleic Acids Research. 2019 ; Vol. 47, No. 13. pp. 7003-7017.

Harvard

Simon, LM, Morandi, E, Luganini, A, Gribaudo, G, Martinez-Sobrido, L, Turner, DH, Oliviero, S & Incarnato, D 2019, 'In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs', Nucleic Acids Research, vol. 47, no. 13, pp. 7003-7017. https://doi.org/10.1093/nar/gkz318

Standard

In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs. / Simon, Lisa Marie; Morandi, Edoardo; Luganini, Anna; Gribaudo, Giorgio; Martinez-Sobrido, Luis; Turner, Douglas H.; Oliviero, Salvatore; Incarnato, Danny.

In: Nucleic Acids Research, Vol. 47, No. 13, 26.07.2019, p. 7003-7017.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Simon LM, Morandi E, Luganini A, Gribaudo G, Martinez-Sobrido L, Turner DH et al. In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs. Nucleic Acids Research. 2019 Jul 26;47(13):7003-7017. https://doi.org/10.1093/nar/gkz318


BibTeX

@article{21979fb9b2b844698bad4605b9cdd4ce,
title = "In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs",
abstract = "The influenza A virus (IAV) is a continuous health threat to humans as well as animals due to its recurring epidemics and pandemics. The IAV genome is segmented and the eight negative-sense viral RNAs (vRNAs) are transcribed into positive sense complementary RNAs (cRNAs) and viral messenger RNAs (mRNAs) inside infected host cells. A role for the secondary structure of IAV mRNAs has been hypothesized and debated for many years, but knowledge on the structure mRNAs adopt in vivo is currently missing. Here we solve, for the first time, the in vivo secondary structure of IAV mRNAs in living infected cells. We demonstrate that, compared to the in vitro refolded structure, in vivo IAV mRNAs are less structured but exhibit specific locally stable elements. Moreover, we show that the targeted disruption of these high-confidence structured domains results in an extraordinary attenuation of IAV replicative capacity. Collectively, our data provide the first comprehensive map of the in vivo structural landscape of IAV mRNAs, hence providing the means for the development of new RNA-targeted antivirals.",
keywords = "REVEALS PRINCIPLES, BALOXAVIR MARBOXIL, VIRAL-RNA, VIRUS, SHAPE, ARCHITECTURE, MECHANISMS, DISCOVERY, SELECTION, ALIGNMENT",
author = "Simon, {Lisa Marie} and Edoardo Morandi and Anna Luganini and Giorgio Gribaudo and Luis Martinez-Sobrido and Turner, {Douglas H.} and Salvatore Oliviero and Danny Incarnato",
year = "2019",
month = jul,
day = "26",
doi = "10.1093/nar/gkz318",
language = "English",
volume = "47",
pages = "7003--7017",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "13",

}

RIS

TY - JOUR

T1 - In vivo analysis of influenza A mRNA secondary structures identifies critical regulatory motifs

AU - Simon, Lisa Marie

AU - Morandi, Edoardo

AU - Luganini, Anna

AU - Gribaudo, Giorgio

AU - Martinez-Sobrido, Luis

AU - Turner, Douglas H.

AU - Oliviero, Salvatore

AU - Incarnato, Danny

PY - 2019/7/26

Y1 - 2019/7/26

N2 - The influenza A virus (IAV) is a continuous health threat to humans as well as animals due to its recurring epidemics and pandemics. The IAV genome is segmented and the eight negative-sense viral RNAs (vRNAs) are transcribed into positive sense complementary RNAs (cRNAs) and viral messenger RNAs (mRNAs) inside infected host cells. A role for the secondary structure of IAV mRNAs has been hypothesized and debated for many years, but knowledge on the structure mRNAs adopt in vivo is currently missing. Here we solve, for the first time, the in vivo secondary structure of IAV mRNAs in living infected cells. We demonstrate that, compared to the in vitro refolded structure, in vivo IAV mRNAs are less structured but exhibit specific locally stable elements. Moreover, we show that the targeted disruption of these high-confidence structured domains results in an extraordinary attenuation of IAV replicative capacity. Collectively, our data provide the first comprehensive map of the in vivo structural landscape of IAV mRNAs, hence providing the means for the development of new RNA-targeted antivirals.

AB - The influenza A virus (IAV) is a continuous health threat to humans as well as animals due to its recurring epidemics and pandemics. The IAV genome is segmented and the eight negative-sense viral RNAs (vRNAs) are transcribed into positive sense complementary RNAs (cRNAs) and viral messenger RNAs (mRNAs) inside infected host cells. A role for the secondary structure of IAV mRNAs has been hypothesized and debated for many years, but knowledge on the structure mRNAs adopt in vivo is currently missing. Here we solve, for the first time, the in vivo secondary structure of IAV mRNAs in living infected cells. We demonstrate that, compared to the in vitro refolded structure, in vivo IAV mRNAs are less structured but exhibit specific locally stable elements. Moreover, we show that the targeted disruption of these high-confidence structured domains results in an extraordinary attenuation of IAV replicative capacity. Collectively, our data provide the first comprehensive map of the in vivo structural landscape of IAV mRNAs, hence providing the means for the development of new RNA-targeted antivirals.

KW - REVEALS PRINCIPLES

KW - BALOXAVIR MARBOXIL

KW - VIRAL-RNA

KW - VIRUS

KW - SHAPE

KW - ARCHITECTURE

KW - MECHANISMS

KW - DISCOVERY

KW - SELECTION

KW - ALIGNMENT

UR - http://www.scopus.com/inward/record.url?scp=85070182306&partnerID=8YFLogxK

U2 - 10.1093/nar/gkz318

DO - 10.1093/nar/gkz318

M3 - Article

C2 - 31053845

AN - SCOPUS:85070182306

VL - 47

SP - 7003

EP - 7017

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 13

ER -

ID: 95845251