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In vitro assays for cobblestone area-forming cells, LTC-IC, and CFU-C

van Os, R. P., Dethmers-Ausema, B. & de Haan, G., 2008, Methods in Molecular Biology: Hematopoietic Stem Cell Protocols. Bunting, K. (ed.). III ed. Humana Press, Vol. 430. p. 143-57 15 p.

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  • Book Chapter In vitro assays van Os, Dethmers-Ausema and de Haan

    Final publisher's version, 594 KB, PDF document

DOI

Various assays exist that measure the function of hematopoietic stemcells (HSCs). In this chapter, in vitro assays are described that measure the frequency of progenitors (colony-forming unit in culture; CFU-C), stem cells (long-term culture-initiating cell; LTC-IC), or both (cobblestone area-forming cell assay; CAFC). These assays measure the potential of a test cell population retrospectively, i.e., at the time its activity is evident when the stem cell itself is often not detectable anymore. Although the in vitro LTC-IC and CAFC assays have been shown to correlate with in vivo activity, in vivo transplantation assays, where it can be shown that cells possess the ability to indefinitely repopulate all blood lineages, are the ultimate proof for HSC activity. Nevertheless, these in vitro assays provide an excellent method to screen for stem cell activity of a putative stem cell population or for screening the effect of a certain treatment on HSCs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationHematopoietic Stem Cell Protocols
EditorsKevin Bunting
PublisherHumana Press
Pages143-57
Number of pages15
Volume430
EditionIII
ISBN (Print)1064-3745
Publication statusPublished - 2008

    Keywords

  • Cell Line, Cell Lineage, Hematopoietic Stem Cells, Humans, Stromal Cells

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