Publication

In Acute Experimental Autoimmune Encephalomyelitis, Infiltrating Macrophages Are Immune Activated, Whereas Microglia Remain Immune Suppressed

Vainchtein, I. D., Vinet, J., Brouwer, N., Brendecke, S., Biagini, G., Biber, K., Boddeke, H. W. G. M. & Eggen, B. J. L., Oct-2014, In : Glia. 62, 10, p. 1724-1735 12 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Vainchtein, I. D., Vinet, J., Brouwer, N., Brendecke, S., Biagini, G., Biber, K., ... Eggen, B. J. L. (2014). In Acute Experimental Autoimmune Encephalomyelitis, Infiltrating Macrophages Are Immune Activated, Whereas Microglia Remain Immune Suppressed. Glia, 62(10), 1724-1735. https://doi.org/10.1002/glia.22711

Author

Vainchtein, I. D. ; Vinet, J. ; Brouwer, N. ; Brendecke, S. ; Biagini, G. ; Biber, K. ; Boddeke, H. W. G. M. ; Eggen, B. J. L. / In Acute Experimental Autoimmune Encephalomyelitis, Infiltrating Macrophages Are Immune Activated, Whereas Microglia Remain Immune Suppressed. In: Glia. 2014 ; Vol. 62, No. 10. pp. 1724-1735.

Harvard

Vainchtein, ID, Vinet, J, Brouwer, N, Brendecke, S, Biagini, G, Biber, K, Boddeke, HWGM & Eggen, BJL 2014, 'In Acute Experimental Autoimmune Encephalomyelitis, Infiltrating Macrophages Are Immune Activated, Whereas Microglia Remain Immune Suppressed', Glia, vol. 62, no. 10, pp. 1724-1735. https://doi.org/10.1002/glia.22711

Standard

In Acute Experimental Autoimmune Encephalomyelitis, Infiltrating Macrophages Are Immune Activated, Whereas Microglia Remain Immune Suppressed. / Vainchtein, I. D.; Vinet, J.; Brouwer, N.; Brendecke, S.; Biagini, G.; Biber, K.; Boddeke, H. W. G. M.; Eggen, B. J. L.

In: Glia, Vol. 62, No. 10, 10.2014, p. 1724-1735.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Vainchtein ID, Vinet J, Brouwer N, Brendecke S, Biagini G, Biber K et al. In Acute Experimental Autoimmune Encephalomyelitis, Infiltrating Macrophages Are Immune Activated, Whereas Microglia Remain Immune Suppressed. Glia. 2014 Oct;62(10):1724-1735. https://doi.org/10.1002/glia.22711


BibTeX

@article{2880f18c65374ec4b754fbe905aed391,
title = "In Acute Experimental Autoimmune Encephalomyelitis, Infiltrating Macrophages Are Immune Activated, Whereas Microglia Remain Immune Suppressed",
abstract = "Multiple sclerosis (MS) is an autoimmune demyelinating disorder of the central nervous system (CNS) characterized by loss of myelin accompanied by infiltration of T-lymphocytes and monocytes. Although it has been shown that these infiltrates are important for the progression of MS, the role of microglia, the resident macrophages of the CNS, remains ambiguous. Therefore, we have compared the phenotypes of microglia and macrophages in a mouse model for MS, experimental autoimmune encephalomyelitis (EAE). In order to properly discriminate between these two cell types, microglia were defined as CD11b(pos) CD45(int) Ly-6C(neg), and infiltrated macrophages as CD11b(pos) CD45(high) Ly-6C(pos). During clinical EAE, microglia displayed a weakly immune-activated phenotype, based on the expression of MHCII, co-stimulatory molecules (CD80, CD86, and CD40) and proinflammatory genes [interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha)]. In contrast, CD11b(pos) CD45(high) Ly6C(pos) infiltrated macrophages were strongly activated and could be divided into two populations Ly-6C(int) and Ly-6C(high), respectively. Ly-6C(high) macrophages contained less myelin than Ly-6C(int) macrophages and expression levels of the proinflammatory cytokines IL-1 beta and TNF-alpha were higher in Ly-6C(int) macrophages. Together, our data show that during clinical EAE, microglia are only weakly activated whereas infiltrated macrophages are highly immune reactive.",
keywords = "microglia, macrophage, Ly-6C, EAE, multiple sclerosis, EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS, CENTRAL-NERVOUS-SYSTEM, MULTIPLE-SCLEROSIS, WHITE-MATTER, CNS, INFLAMMATION, MONOCYTES, BRAIN, PATHOGENESIS, RECEPTOR",
author = "Vainchtein, {I. D.} and J. Vinet and N. Brouwer and S. Brendecke and G. Biagini and K. Biber and Boddeke, {H. W. G. M.} and Eggen, {B. J. L.}",
year = "2014",
month = "10",
doi = "10.1002/glia.22711",
language = "English",
volume = "62",
pages = "1724--1735",
journal = "Glia",
issn = "0894-1491",
publisher = "WILEY",
number = "10",

}

RIS

TY - JOUR

T1 - In Acute Experimental Autoimmune Encephalomyelitis, Infiltrating Macrophages Are Immune Activated, Whereas Microglia Remain Immune Suppressed

AU - Vainchtein, I. D.

AU - Vinet, J.

AU - Brouwer, N.

AU - Brendecke, S.

AU - Biagini, G.

AU - Biber, K.

AU - Boddeke, H. W. G. M.

AU - Eggen, B. J. L.

PY - 2014/10

Y1 - 2014/10

N2 - Multiple sclerosis (MS) is an autoimmune demyelinating disorder of the central nervous system (CNS) characterized by loss of myelin accompanied by infiltration of T-lymphocytes and monocytes. Although it has been shown that these infiltrates are important for the progression of MS, the role of microglia, the resident macrophages of the CNS, remains ambiguous. Therefore, we have compared the phenotypes of microglia and macrophages in a mouse model for MS, experimental autoimmune encephalomyelitis (EAE). In order to properly discriminate between these two cell types, microglia were defined as CD11b(pos) CD45(int) Ly-6C(neg), and infiltrated macrophages as CD11b(pos) CD45(high) Ly-6C(pos). During clinical EAE, microglia displayed a weakly immune-activated phenotype, based on the expression of MHCII, co-stimulatory molecules (CD80, CD86, and CD40) and proinflammatory genes [interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha)]. In contrast, CD11b(pos) CD45(high) Ly6C(pos) infiltrated macrophages were strongly activated and could be divided into two populations Ly-6C(int) and Ly-6C(high), respectively. Ly-6C(high) macrophages contained less myelin than Ly-6C(int) macrophages and expression levels of the proinflammatory cytokines IL-1 beta and TNF-alpha were higher in Ly-6C(int) macrophages. Together, our data show that during clinical EAE, microglia are only weakly activated whereas infiltrated macrophages are highly immune reactive.

AB - Multiple sclerosis (MS) is an autoimmune demyelinating disorder of the central nervous system (CNS) characterized by loss of myelin accompanied by infiltration of T-lymphocytes and monocytes. Although it has been shown that these infiltrates are important for the progression of MS, the role of microglia, the resident macrophages of the CNS, remains ambiguous. Therefore, we have compared the phenotypes of microglia and macrophages in a mouse model for MS, experimental autoimmune encephalomyelitis (EAE). In order to properly discriminate between these two cell types, microglia were defined as CD11b(pos) CD45(int) Ly-6C(neg), and infiltrated macrophages as CD11b(pos) CD45(high) Ly-6C(pos). During clinical EAE, microglia displayed a weakly immune-activated phenotype, based on the expression of MHCII, co-stimulatory molecules (CD80, CD86, and CD40) and proinflammatory genes [interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha)]. In contrast, CD11b(pos) CD45(high) Ly6C(pos) infiltrated macrophages were strongly activated and could be divided into two populations Ly-6C(int) and Ly-6C(high), respectively. Ly-6C(high) macrophages contained less myelin than Ly-6C(int) macrophages and expression levels of the proinflammatory cytokines IL-1 beta and TNF-alpha were higher in Ly-6C(int) macrophages. Together, our data show that during clinical EAE, microglia are only weakly activated whereas infiltrated macrophages are highly immune reactive.

KW - microglia

KW - macrophage

KW - Ly-6C

KW - EAE

KW - multiple sclerosis

KW - EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS

KW - CENTRAL-NERVOUS-SYSTEM

KW - MULTIPLE-SCLEROSIS

KW - WHITE-MATTER

KW - CNS

KW - INFLAMMATION

KW - MONOCYTES

KW - BRAIN

KW - PATHOGENESIS

KW - RECEPTOR

U2 - 10.1002/glia.22711

DO - 10.1002/glia.22711

M3 - Article

VL - 62

SP - 1724

EP - 1735

JO - Glia

JF - Glia

SN - 0894-1491

IS - 10

ER -

ID: 14070706