Publication

Improving the reproducibility of hydrogel-coated glutamate microsensors by using an automated dipcoater

Oldenziel, W. H., Beukema, W. & Westerink, B. H. C., 30-Dec-2004, In : Journal of Neuroscience Methods. 140, 1-2, p. 117 - 126 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Weite Hendrik Oldenziel
  • W. Beukema
  • B.H.C. Westerink

Hydrogel-coated microsensors based oil carbon fiber electrodes (CFEs) are promising tools for in vivo analysis of endogeneous compounds such as glutamate. However. their construction generally depends on manual fabrication. which often results in pool reproducibility. The aim of this study was to improve the reproducibility and performance of glutamate microsensors. CFEs (10 mum diameter. 300-500 mum long) were coated with a cross-linked redox-polymer hydrogel containing L-glutamate oxidase, horseradish peroxidase and ascorbate oxidase. Various parameters that are likely to influence the reproducibility of the glutamate microsensors were studied. It appeared that the most crucial step in determining the microsensor performance is the manual hydrogel-application procedure. To control this procedure an automated dipcoater was constructed, which allowed mechanical application of the hydrogel on the CFE under standardized conditions. Significant improvements in performance were seen when the CFEs were dipcoated for 10 min at 37 degreesC. Further improvements were obtained when the automated hydrogel application was combined with other cross-link methods. such as electrodeposition and electrostatic complexation. A crucial factor in determining the microsensor performance is the hydrogel thickness. Microscopic observations revealed that, despite the use of an automated dipcoater, the layer thickness was not constant. By conbining the automated dipcoat technique with amperometry, the layer thickness could be indirectly monitored and controlled, which resulted ill significant improvements of the reproducibility of the sensors. (C) 2004 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)117 - 126
Number of pages10
JournalJournal of Neuroscience Methods
Volume140
Issue number1-2
Publication statusPublished - 30-Dec-2004
Event10th International Conference on In Vivo Methods - , Sweden
Duration: 24-Jun-200327-Jun-2003

Event

10th International Conference on In Vivo Methods

24/06/200327/06/2003

Sweden

Event: Other

    Keywords

  • CFE, hydrogel, L-glutamate oxidase, microsensor, construction, L-glutamate, LAYER-BY-LAYER, IN-VIVO, BRAIN MICRODIALYSIS, CARBON ELECTRODES, RELEASE, GLUCOSE, SENSORS, MICROELECTRODE, ASCORBATE, OXIDASE

ID: 1246217