Improving selectivity and sensitivity of protein quantitation by LC-HR-MS/MS: Determination of somatropin in rat plasmaBults, P., Meints, M., Sonesson, A., Knutsson, M., Bischoff, R. & van de Merbel, N. C., 18-Jul-2018, In : Bioanalysis. 10, 13, p. 1009-1021
Research output: Contribution to journal › Article › Academic › peer-review
AIM: Protein quantitation by digestion of a biological sample followed by LC-MS analysis of a signature peptide can be a challenge because of the high complexity of the digested matrix. Results/methodology: The use of LC with high-resolution (quadrupole-TOF) MS detection allowed quantitation of the 22-kDa biopharmaceutical somatropin in 60 μl of rat plasma down to 25 ng/ml with minimal further sample treatment. Reducing the mass extraction window to 0.01 Da considerably decreased the interference of tryptic peptides, enhanced sensitivity and improved accuracy and precision. Analysis with LC-MS/MS resulted in a less favorable limit of quantitation of 100 ng/ml.
CONCLUSION: HRMS is an interesting option for the quantitation of proteins after digestion and has the potential to improve sensitivity with minimal method development.
|Early online date||28-Jun-2018|
|Publication status||Published - 18-Jul-2018|